Anti-GPBB antibody - N-terminal (ab228884)
Key features and details
- Rabbit polyclonal to GPBB - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GPBB antibody - N-terminal
See all GPBB primary antibodies -
Description
Rabbit polyclonal to GPBB - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Cow, Zebrafish, Rhesus monkey -
Immunogen
Recombinant fragment within Human GPBB (N terminal). The exact sequence is proprietary.
Database link: P11216 -
Positive control
- WB: Raji whole cell lysate; mouse brain whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 96 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 96 kDa. |
Target
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Function
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. -
Sequence similarities
Belongs to the glycogen phosphorylase family. -
Post-translational
modificationsPhosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A. - Information by UniProt
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Database links
- Entrez Gene: 421248 Chicken
- Entrez Gene: 5834 Human
- Entrez Gene: 110078 Mouse
- Entrez Gene: 25739 Rat
- Omim: 138550 Human
- SwissProt: P11216 Human
- SwissProt: Q8CI94 Mouse
- SwissProt: P53534 Rat
see all -
Alternative names
- Brain glycogen phosphorylase antibody
- Glycogen phosphorylase B antibody
- Glycogen phosphorylase brain form antibody
see all
Images
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Anti-GPBB antibody - N-terminal (ab228884) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 96 kDa7.5% SDS-PAGE
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Anti-GPBB antibody - N-terminal (ab228884) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 96 kDa7.5% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228884 has not yet been referenced specifically in any publications.