Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab42457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionFunctions as a GC-rich promoter-specific transactivating transcription factor.
Tissue specificityWidely expressed. Some isoforms may be specifically expressed in veins and arteries (at protein level). Isoform 4 is widely expressed. Isoform 1, isoform 2 and isoform 3 may be specifically expressed in vascular smooth muscle cells.
Sequence similaritiesBelongs to the vasculin family.
Cellular localizationNucleus. Cytoplasm. According to PubMed:12842993, it localizes to the cytoplasm.
- Information by UniProt
- DKFZp761C169 antibody
- GC rich promoter binding protein 1 antibody
- GC-rich promoter-binding protein 1 antibody
Anti-GPBP1 antibody (ab42457) at 0.5 µg/ml + HepG2 Cell Lysate
HRP conjugated anti-Rabbit IgG at 1/75000 dilution
Predicted band size: 53 kDa
Observed band size: 52,59 kDa why is the actual band size different from the predicted?
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded Human Kidney sections) using ab42457 at 4-8 ug/ml. Positively labelled epithelial cells of tubule are indicated using arrows.
ab42457 has not yet been referenced specifically in any publications.