Overview

  • Product name

    Anti-GPCR GPR101 antibody
  • Description

    Rabbit polyclonal to GPCR GPR101
  • Host species

    Rabbit
  • Specificity

    ab131117 detects endogenous levels of total GPCR GPR101 protein.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, African green monkey
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from the C-terminus of Human GPCR GPR101.

  • Positive control

    • Human kidney tissue; HeLa cells; COS7 cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tubules labelling GPCR GPR101 with ab131117 at 10 µg/ml.
  • Immunofluorescence analysis of HeLa cells labelling GPCR GPR101 with ab131117 at a 1/100 dilution. The image on the right is treated with the synthesized peptide.
  • All lanes : Anti-GPCR GPR101 antibody (ab131117) at 1/500 dilution

    Lane 1 : COS7 cell extract
    Lane 2 : COS7 cell extract with synthesized peptide

    Predicted band size: 57 kDa

References

ab131117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131117.
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