Overview

  • Product name

    Anti-GPCR GPR115 antibody - C-terminal
  • Description

    Rabbit polyclonal to GPCR GPR115 - C-terminal
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except GPR113 (69%).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Monkey, Gorilla, Common marmoset, Elephant
  • Immunogen

    A 16 amino acid synthetic peptide corresponding to the C-terminus of Human GPCR GPR115 (NP_722580.3).

  • Positive control

    • Human breast, epithelium tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab150485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Orphan receptor.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ38076 antibody
    • G protein coupled receptor 115 antibody
    • G protein coupled receptor PGR18 antibody
    • G-protein coupled receptor PGR18 antibody
    • GP115_HUMAN antibody
    • GPR115 antibody
    • PGR18 antibody
    • Probable G protein coupled receptor 115 antibody
    • Probable G-protein coupled receptor 115 antibody
    • Seven transmembrane helix receptor antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human breast, epithelium tissue labelling GPCR GPR115 with ab150485 antibody at a concentration of 10 µg/ml.

References

ab150485 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9
Permeabilization
No
Specification
Skin
Blocking step
hydrogen peroxide as blocking agent for 30 minute(s) · Concentration: 0.3% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 06 2018

Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Cos-7)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
30 µg
Treatment
transfected with HA-GPR115 pcDNA 3.1
Specification
Cos-7
Blocking step
Odyssey TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.05% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 21 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH9
Permeabilization
No
Specification
Pancreas
Blocking step
(agent) for 30 minute(s) · Concentration: 0.3% · Temperature: 22°C
Fixative
Formaldehyde

Romy Winkler

Verified customer

Submitted Mar 19 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Pancreas)
Permeabilization
No
Specification
Pancreas
Blocking step
hydrogen peroxide as blocking agent for 30 minute(s) · Concentration: 0.3% · Temperature: 22°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Mar 19 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Cos-7)
Permeabilization
No
Specification
Cos-7
Fixative
Acetone

Abcam user community

Verified customer

Submitted Mar 14 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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