Key features and details
- Rabbit polyclonal to GPCR GPR116
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GPCR GPR116 antibody
See all GPCR GPR116 primary antibodies
DescriptionRabbit polyclonal to GPCR GPR116
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human breast carcinoma tissue; A549 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111169 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab111169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionMay have a role in the regulation of acid-base balance.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 3 Ig-like (immunoglobulin-like) domains.
Contains 1 SEA domain.
modificationsProteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked.
Cellular localizationCell membrane.
- Information by UniProt
- DKFZp564O1923 antibody
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- G protein coupled receptor 116 antibody
ab111169 at 1/50 dilution staining GPCR GPR116 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized peptide.
ab111169 at 1/100 dilution staining GPCR GPR116 in A549 cells by Immunofluorescence. The image on the right is treated with synthesized peptide.
ab111169 has been referenced in 1 publication.
- Tang X et al. GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the Gaq-p63RhoGEF-Rho GTPase pathway. Cancer Res 73:6206-18 (2013). IHC ; Human . PubMed: 24008316