Key features and details
- Rabbit polyclonal to GPCR GPR120
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GPCR GPR120 antibody
See all GPCR GPR120 primary antibodies
DescriptionRabbit polyclonal to GPCR GPR120
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human GPCR GPR120 aa 226-284. Synthetic peptide around Val 259.
Database link: Q5NUL3
- IHC-P: Human testis tissue. WB: HEK-293T, Raw 264.7 and PC-12 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 42 kDa.|
FunctionReceptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects particulary in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediates the taste of fatty acids.
Tissue specificityAbundant expression in the intestinal tract.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPhosphorylated. FFA stimulation facilitates phosphorylation.
Cellular localizationCell membrane. Colocalized with ARRB2 following DHA treatment.
- Information by UniProt
- BMIQ10 antibody
- FFAR4 antibody
- Free fatty acid receptor 4 antibody
All lanes : Anti-GPCR GPR120 antibody (ab230869) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
Lane 2 : Raw 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate.
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate.
Predicted band size: 42 kDa
Formalin-fixed, paraffin-embedded human testis tissue stained for GPCR GPR120 using ab230869 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230869 has not yet been referenced specifically in any publications.