Product nameAnti-GPCR GPR120 antibody
See all GPCR GPR120 primary antibodies
DescriptionRabbit polyclonal to GPCR GPR120
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Synthetic peptide corresponding to Mouse GPCR GPR120 (N terminal).
Database link: Q7TMA4
- WB: RAW 264.7, NIH/3T3 and PC-12 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab263964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.|
FunctionReceptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects particulary in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediates the taste of fatty acids.
Tissue specificityAbundant expression in the intestinal tract.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPhosphorylated. FFA stimulation facilitates phosphorylation.
Cellular localizationCell membrane. Colocalized with ARRB2 following DHA treatment.
- Information by UniProt
- BMIQ10 antibody
- FFAR4 antibody
- Free fatty acid receptor 4 antibody
All lanes : Anti-GPCR GPR120 antibody (ab263964) at 1/1000 dilution (overnight at 4degC)
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-Rabbit HRP secondary antibody for 30 min at RT at 1/70000 dilution
Predicted band size: 42 kDa
Additional bands at: 48 kDa (possible glycosylated form)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263964 has not yet been referenced specifically in any publications.