Anti-GPCR GPR124 antibody (ab67280)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-GPCR GPR124 antibody
See all GPCR GPR124 primary antibodies -
Description
Rabbit polyclonal to GPCR GPR124 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide (Human) of 14 amino acids from the central region
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Positive control
- Rat Kidney Tissue Lysate Human bladder tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 142 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube. -
Tissue specificity
Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Domain
Binds to DLG1 through its PDZ-binding motif. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 25960 Human
- Entrez Gene: 306543 Rat
- Omim: 606823 Human
- SwissProt: Q96PE1 Human
- Unigene: 274136 Human
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Alternative names
- DKFZp434C211 antibody
- DKFZp434J0911 antibody
- FLJ14390 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR124 antibody (ab67280)Immunofluorescence of GPCR GPR124 in Human bladder tissue with ab67280 at 20 ug/mL.
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Western blot - Anti-GPCR GPR124 antibody (ab67280)Lane 1 : Anti-GPCR GPR124 antibody (ab67280) at 2 µg/ml
Lane 2 : Anti-GPCR GPR124 antibody (ab67280) at 4 µg/ml
All lanes : Rat kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 142 kDa
Observed band size: 176,180 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (ab67280)ab67280 at 5µg/ml staining of GPCR GPR124 in human bladder tissue using IHC-P.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang Z et al. ß-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells. Oncol Lett 16:2143-2150 (2018). Read more (PubMed: 30008912) »
