Anti-GPCR GPR124 antibody (ab67280)
Key features and details
- Rabbit polyclonal to GPCR GPR124
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GPCR GPR124 antibody
See all GPCR GPR124 primary antibodies -
Description
Rabbit polyclonal to GPCR GPR124 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide (Human) of 14 amino acids from the central region
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Positive control
- Rat Kidney Tissue Lysate Human bladder tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab67280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 142 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Function
Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube. -
Tissue specificity
Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Domain
Binds to DLG1 through its PDZ-binding motif. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 25960 Human
- Entrez Gene: 306543 Rat
- Omim: 606823 Human
- SwissProt: Q96PE1 Human
- Unigene: 274136 Human
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Alternative names
- DKFZp434C211 antibody
- DKFZp434J0911 antibody
- FLJ14390 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR124 antibody (ab67280)Immunofluorescence of GPCR GPR124 in Human bladder tissue with ab67280 at 20 ug/mL.
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Western blot - Anti-GPCR GPR124 antibody (ab67280)Lane 1 : Anti-GPCR GPR124 antibody (ab67280) at 2 µg/ml
Lane 2 : Anti-GPCR GPR124 antibody (ab67280) at 4 µg/ml
All lanes : Rat kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 142 kDa
Observed band size: 176,180 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (ab67280)ab67280 at 5µg/ml staining of GPCR GPR124 in human bladder tissue using IHC-P.
Protocols
Datasheets and documents
References (1)
ab67280 has been referenced in 1 publication.
- Wang Z et al. ß-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells. Oncol Lett 16:2143-2150 (2018). PubMed: 30008912
