Overview

  • Product name

  • Description

    Rabbit polyclonal to GPCR GPR124
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 14 amino acids from the central region

  • Positive control

    • Rat Kidney Tissue Lysate Human bladder tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab67280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 142 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube.
  • Tissue specificity

    Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Domain

    Binds to DLG1 through its PDZ-binding motif.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp434C211 antibody
    • DKFZp434J0911 antibody
    • FLJ14390 antibody
    • G protein coupled receptor 124 antibody
    • G-protein coupled receptor 124 antibody
    • GP124_HUMAN antibody
    • GPR 124 antibody
    • Gpr124 antibody
    • KIAA1531 antibody
    • TEM 5 antibody
    • TEM5 antibody
    • Tumor endothelial marker 5 antibody
    • Tumor endothelial marker 5 precursor antibody
    see all

Images

  • Immunofluorescence of GPCR GPR124 in Human bladder tissue with ab67280 at 20 ug/mL.

  • Lane 1 : Anti-GPCR GPR124 antibody (ab67280) at 2 µg/ml
    Lane 2 : Anti-GPCR GPR124 antibody (ab67280) at 4 µg/ml

    All lanes : Rat kidney tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 142 kDa
    Observed band size: 176,180 kDa
    why is the actual band size different from the predicted?

  • ab67280 at 5µg/ml staining of  GPCR GPR124 in human bladder tissue using IHC-P.

References

This product has been referenced in:

  • Wang Z  et al. ß-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells. Oncol Lett 16:2143-2150 (2018). Read more (PubMed: 30008912) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab67280.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up