Overview

  • Product name
    Anti-GPCR GPR124 antibody
    See all GPCR GPR124 primary antibodies
  • Description
    Rabbit polyclonal to GPCR GPR124
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 14 amino acids from the central region

  • Positive control
    • Rat Kidney Tissue Lysate Human bladder tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab67280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 142 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Endothelial receptor that acts as an essential regulator of CNS angiogenesis. Required for normal endothelial cell sprouting and migration in the forebrain and neural tube.
  • Tissue specificity
    Expressed in endothelial cells (at protein level). Abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
    Contains 1 GPS domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Domain
    Binds to DLG1 through its PDZ-binding motif.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • DKFZp434C211 antibody
      • DKFZp434J0911 antibody
      • FLJ14390 antibody
      • G protein coupled receptor 124 antibody
      • G-protein coupled receptor 124 antibody
      • GP124_HUMAN antibody
      • GPR 124 antibody
      • Gpr124 antibody
      • KIAA1531 antibody
      • TEM 5 antibody
      • TEM5 antibody
      • Tumor endothelial marker 5 antibody
      • Tumor endothelial marker 5 precursor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR124 antibody (ab67280)
      Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR124 antibody (ab67280)

      Immunofluorescence of GPCR GPR124 in Human bladder tissue with ab67280 at 20 ug/mL.

    • Western blot - Anti-GPCR GPR124 antibody (ab67280)
      Western blot - Anti-GPCR GPR124 antibody (ab67280)
      Lane 1 : Anti-GPCR GPR124 antibody (ab67280) at 2 µg/ml
      Lane 2 : Anti-GPCR GPR124 antibody (ab67280) at 4 µg/ml

      All lanes : Rat kidney tissue lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 142 kDa
      Observed band size: 176,180 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (ab67280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR124 antibody (ab67280)
      ab67280 at 5µg/ml staining of  GPCR GPR124 in human bladder tissue using IHC-P.

    References

    ab67280 has not yet been referenced specifically in any publications.

    Publishing research using ab67280? Please let us know so that we can cite the reference in this datasheet.

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