Overview

  • Product name

  • Description

    Rabbit polyclonal to GPCR GPR125
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    TKRGNNTRTVDIVVLESSAQYCPPERV

    conjugated to KLH, corresponding to internal sequence amino acids 328-354 of Human GPCR GPR125

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
ELISA 1/5000.
IHC-Fr 1/100.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    GPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.
  • Cellular localization

    Cell membrane; Multi pass membrane protein.
  • Database links

  • Alternative names

    • FLJ38547 antibody
    • G protein coupled receptor 125 antibody
    • PGR21 antibody
    • Probable G protein coupled receptor 125 precursor antibody
    • TEM5 like antibody
    • TEM5L antibody
    see all

Images

  • ICC/IF image of ab51705 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51705, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Wang H  et al. Transcriptional regulation of P63 on the apoptosis of male germ cells and three stages of spermatogenesis in mice. Cell Death Dis 9:76 (2018). ICC/IF . Read more (PubMed: 29362488) »
  • Fu H  et al. PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways. Mol Ther Nucleic Acids 12:769-786 (2018). Read more (PubMed: 30141410) »
See all 12 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us again.

I have reviewed this case again, and it appears that WB was supposed to be removed as a tested application a while ago. However, this was impossible because of a programming error on ourinternal datasheetsite.



I deeply apologize to your customer and I am more than happy to send him credit note or free of charge replacement of his choice.


I hope this information is helpful to you.I am looking forward to your reply.

Read More

Question
Answer

Pour bénéficier des frais de port gratuits sur votre prochaine commande de ab51705, merci d'indiquer sur votre bon de commande la note suivante : "Frais de port offerts, code ********".

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line(MDA-MB-231))
Loading amount
20 µg
Specification
breast cancer cell line(MDA-MB-231)
Gel Running Conditions
Reduced Denaturing (8% stacking gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Wang xiu

Verified customer

Submitted Jun 09 2008

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