Product nameAnti-GPCR GPR125 antibody
See all GPCR GPR125 primary antibodies
DescriptionGoat polyclonal to GPCR GPR125
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- Human ovary lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab82145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 146 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceGPR125 is an orphan receptor which has a leucine rich repeat (LRR),an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin andtitin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion,connective tissue, ear, embryo, eye,ganglion, heart, lung,placenta, and skin libraries.
Cellular localizationCell membrane; Multi pass membrane protein.
- FLJ38547 antibody
- G protein coupled receptor 125 antibody
- PGR21 antibody
Anti-GPCR GPR125 antibody (ab82145) at 0.5 µg/ml + human ovary lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 146 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 33 kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab82145 has not yet been referenced specifically in any publications.