Key features and details
- Rabbit polyclonal to GPCR GPR20
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GPCR GPR20 antibody
See all GPCR GPR20 primary antibodies
DescriptionRabbit polyclonal to GPCR GPR20
Specificityab75559 detects endogenous levels of total GPCR GPR20 protein.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthesized peptide derived from an internal sequence of human GPCR GPR20
- IHC-P: human brain tissue WB: extracts from LOVO cells ICC/IF: A549 cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab75559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 38 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionOrphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
Tissue specificityUbiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- G protein coupled receptor 20 antibody
- G-protein coupled receptor 20 antibody
- Gpr20 antibody
- GPR20_HUMAN antibody
All lanes : Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilution
Lane 1 : extracts from LOVO cells
Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
ab75559 has not yet been referenced specifically in any publications.