Product nameAnti-GPCR GPR32 antibody
See all GPCR GPR32 primary antibodies
DescriptionRabbit polyclonal to GPCR GPR32
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human GPCR GPR32 (extracellular).
- A549 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/1000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/500 - 1/1000.|
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- G protein coupled receptor GPR32 antibody
- GPR32 antibody
- GPR32_HUMAN antibody
ab79516 staining GPCR GPR32 in Human primary small airway epithelial cells by Flow Cytometry. Cells were fixed with formaldehyde and incubated with the primary antibody (1/250) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.
Gating Strategy: Forward and side scatter.
All lanes : Anti-GPCR GPR32 antibody (ab79516) at 1/500 dilution
Lane 1 : Extracts from A549 cells
Lane 2 : Extracts from A549 cells with immunizing peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 40 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Left panel: immunofluorescence analysis of A549 cells using ab79516 at a 1/500 dilution. Right panel: same, after pre-incubation with the immunizing peptide.
This product has been referenced in:
- Lannan KL et al. Maresin 1 induces a novel pro-resolving phenotype in human platelets. J Thromb Haemost 15:802-813 (2017). Read more (PubMed: 28079976) »
- Hsiao HM et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »