Overview

  • Product name

  • Description

    Rabbit polyclonal to GPCR GPR32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GPCR GPR32 (extracellular).

  • Positive control

    • A549 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/250.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

Images

  • ab79516 staining GPCR GPR32 in Human primary small airway epithelial cells by Flow Cytometry. Cells were fixed with formaldehyde and incubated with the primary antibody (1/250) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.
    Gating Strategy: Forward and side scatter.

    See Abreview

  • All lanes : Anti-GPCR GPR32 antibody (ab79516) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from A549 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

  • Left panel: immunofluorescence analysis of A549 cells using ab79516 at a 1/500 dilution. Right panel: same, after pre-incubation with the immunizing peptide.

References

This product has been referenced in:

  • Lannan KL  et al. Maresin 1 induces a novel pro-resolving phenotype in human platelets. J Thromb Haemost 15:802-813 (2017). Read more (PubMed: 28079976) »
  • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Fixation
Formaldehyde
Sample
Human Cell (Human Primary Small Airway epithelial cell)
Specification
Human Primary Small Airway epithelial cell
Gating Strategy
Forward and side scatter
Permeabilization
No

Dr. Jim Hsiao

Verified customer

Submitted May 15 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Human Primary Lung Fibroblasts)
Specification
Human Primary Lung Fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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