Anti-GPCR GPR34 antibody (ab134811)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GPCR GPR34 antibody
See all GPCR GPR34 primary antibodies -
Description
Rabbit polyclonal to GPCR GPR34 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide derived from internal sequence of Human GPCR GPR34.
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Positive control
- IHC-P: Human Spleen tissue; WB: HT-29, K562, and COS7 cell extracts; IF: LOVO cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+, Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 44 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Relevance
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR34 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, particularly in caudate, frontal cortex, putamen, and thalamus, as well as in eye, liver, and testis. ESTs have been isolated from adipose, B-cell/lung/testis, eye, liver/spleen, placenta, tonsil, and uterus libraries. -
Cellular localization
Cell membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 2857 Human
- Entrez Gene: 23890 Mouse
- Entrez Gene: 554353 Rat
- Omim: 300241 Human
- SwissProt: Q9UPC5 Human
- SwissProt: Q9R1K6 Mouse
- Unigene: 495989 Human
- Unigene: 487326 Mouse
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Alternative names
- G protein coupled receptor 34 antibody
- GPCR 34 antibody
- GPCR34 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR34 antibody (ab134811)Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Spleen tissue labelling GPCR GPR34 with ab134811 at 3.75 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR34 antibody (ab134811)Immunofluorescence analysis of LOVO cells labelling GPCR GPR34 with ab134811 at 1/100. Right panel represents cells treated with the synthesized peptide. -
Western blot - Anti-GPCR GPR34 antibody (ab134811)All lanes : Anti-GPCR GPR34 antibody (ab134811) at 1/500 dilution
Lane 1 : HT-29 cell extract
Lane 2 : K562 cell extract
Lane 3 : COS7 cell extract
Lane 4 : HT-29 cell extract treated with the synthesized peptide
Predicted band size: 44 kDa
Protocols
Datasheets and documents
References
ab134811 has not yet been referenced specifically in any publications.
