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    gpcr-gpr34-antibody-ab169455.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-GPCR GPR34 antibody (ab169455)

  • Datasheet
  • SDS
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Western blot - Anti-GPCR GPR34 antibody (ab169455)
  • Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR34 antibody (ab169455)

Key features and details

  • Mouse polyclonal to GPCR GPR34
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human GPCR GPR34 protein (ab158568)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-GPCR GPR34 antibody
    See all GPCR GPR34 primary antibodies
  • Description

    Mouse polyclonal to GPCR GPR34
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Gorilla
  • Immunogen

    Full length protein, corresponding to amino acids 1-381 of Human GPCR GPR34 (AAH20678.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • GPCR GPR34 transfected 293T cell lysate; 293 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Recombinant Protein

    • Recombinant Human GPCR GPR34 protein (ab158568)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Relevance

    G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR34 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, particularly in caudate, frontal cortex, putamen, and thalamus, as well as in eye, liver, and testis. ESTs have been isolated from adipose, B-cell/lung/testis, eye, liver/spleen, placenta, tonsil, and uterus libraries.
  • Cellular localization

    Cell membrane; Multi-pass membrane protein.
  • Database links

    • Entrez Gene: 473572 Chimpanzee
    • Entrez Gene: 2857 Human
    • Entrez Gene: 23890 Mouse
    • Omim: 300241 Human
    • SwissProt: P60019 Chimpanzee
    • SwissProt: Q6XCF2 Gorilla
    • SwissProt: Q9UPC5 Human
    • SwissProt: Q9R1K6 Mouse
    • Unigene: 495989 Human
    • Unigene: 487326 Mouse
    see all
  • Alternative names

    • G protein coupled receptor 34 antibody
    • GPCR 34 antibody
    • GPCR34 antibody
    • GPR 34 antibody
    • GPR34 antibody
    • OTTHUMP00000023148 antibody
    • OTTHUMP00000023149 antibody
    • OTTMUSP00000018373 antibody
    • Probable G protein coupled receptor 34 antibody
    see all

Images

  • Western blot - Anti-GPCR GPR34 antibody (ab169455)
    Western blot - Anti-GPCR GPR34 antibody (ab169455)
    All lanes : Anti-GPCR GPR34 antibody (ab169455) at 1 µg/ml

    Lane 1 : GPCR GPR34 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR34 antibody (ab169455)
    Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR34 antibody (ab169455)
    Immunofluorescent analysis of permeabilized 293 cells labeling GPCR GPR3 with ab169455 at 1µg/ml.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab169455? Please let us know so that we can cite the reference in this datasheet.

ab169455 has been referenced in 1 publication.

  • Pan RY  et al. Sodium rutin ameliorates Alzheimer's disease-like pathology by enhancing microglial amyloid-ß clearance. Sci Adv 5:eaau6328 (2019). PubMed: 30820451

Customer reviews and Q&As

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