Anti-GPCR GPR35 antibody (ab150635)
Key features and details
- Rabbit polyclonal to GPCR GPR35
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPCR GPR35 antibody
See all GPCR GPR35 primary antibodies -
Description
Rabbit polyclonal to GPCR GPR35 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins. -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey, Gorilla
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Immunogen
Synthetic peptide containing 19 amino acids from the C terminus of Human GPCR GPR35 (UniProt ID: Q9HC97).
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Positive control
- Human pancreas tissue; HEK293 Human embryonic kidney cells transfected with GPCR GPR35
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 12 - 23 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use a concentration of 12 - 23 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC
Use at an assay dependent concentration. |
Target
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Relevance
G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR35 is a member of this family and is an orphan receptor. GPR35 expression was reported only in rat intestine (O'Dowd et al., 1998) by Northern hybridization; however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach. -
Cellular localization
Cell membrane; Multi-pass membrane protein -
Database links
- Entrez Gene: 2859 Human
- Omim: 602646 Human
- SwissProt: Q9HC97 Human
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Alternative names
- G protein coupled receptor 35 antibody
- G protein coupled receptor 35 antibody
- GPR 35 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR35 antibody (ab150635)Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling GPCR GPR35 with ab150635 at 23 µg/ml after heat induced antigen retrieval.
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Immunocytochemistry - Anti-GPCR GPR35 antibody (ab150635)Immunocytochemical analysis of GPCR GPR35 transfected HEK293 Human embryonic kidney cells labeling GPCR GPR35 with ab150635.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150635 has been referenced in 1 publication.
- Wang W et al. Overexpression of GPR35 confers drug resistance in NSCLC cells by ß-arrestin/Akt signaling. Onco Targets Ther 11:6249-6257 (2018). PubMed: 30288060