- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-GPCR GPR35 antibody
See all GPCR GPR35 primary antibodies -
DescriptionRabbit polyclonal to GPCR GPR35
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Host speciesRabbit
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SpecificityBLAST analysis of the peptide immunogen showed no homology with other Human proteins.
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Tested applicationsSuitable for: IHC-P, ICCmore details
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Species reactivityReacts with: Rat, Human
Predicted to work with: Monkey, Gorilla
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Immunogen
Synthetic peptide containing 19 amino acids from the C terminus of Human GPCR GPR35 (UniProt ID: Q9HC97).
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Positive control
- Human pancreas tissue; HEK293 Human embryonic kidney cells transfected with GPCR GPR35
Properties
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FormLiquid
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Storage instructionsShipped at 4°C.
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Storage bufferPreservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab150635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 12 - 23 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | Use at an assay dependent concentration. |
Target
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RelevanceG protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR35 is a member of this family and is an orphan receptor. GPR35 expression was reported only in rat intestine (O'Dowd et al., 1998) by Northern hybridization; however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach.
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Cellular localizationCell membrane; Multi-pass membrane protein
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Database links
- Entrez Gene: 2859 Human
- Entrez Gene: 367315 Rat
- Omim: 602646 Human
- SwissProt: Q9HC97 Human
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Alternative names
- G protein coupled receptor 35 antibody
- G protein coupled receptor 35 antibody
- GPR 35 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR35 antibody (ab150635)Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling GPCR GPR35 with ab150635 at 23 µg/ml after heat induced antigen retrieval. -
Immunocytochemistry - Anti-GPCR GPR35 antibody (ab150635)Immunocytochemical analysis of GPCR GPR35 transfected HEK293 Human embryonic kidney cells labeling GPCR GPR35 with ab150635.
References
ab150635 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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