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    gpcr-gpr35-antibody-ab150635.pdf

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Anti-GPCR GPR35 antibody (ab150635)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR35 antibody (ab150635)
  • Immunocytochemistry - Anti-GPCR GPR35 antibody (ab150635)

Key features and details

  • Rabbit polyclonal to GPCR GPR35
  • Suitable for: IHC-P, ICC
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-GPCR GPR35 antibody
    See all GPCR GPR35 primary antibodies
  • Description

    Rabbit polyclonal to GPCR GPR35
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey, Gorilla
  • Immunogen

    Synthetic peptide containing 19 amino acids from the C terminus of Human GPCR GPR35 (UniProt ID: Q9HC97).

  • Positive control

    • Human pancreas tissue; HEK293 Human embryonic kidney cells transfected with GPCR GPR35
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 12 - 23 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.
Notes
IHC-P
Use a concentration of 12 - 23 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
Use at an assay dependent concentration.

Target

  • Relevance

    G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GPR35 is a member of this family and is an orphan receptor. GPR35 expression was reported only in rat intestine (O'Dowd et al., 1998) by Northern hybridization; however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach.
  • Cellular localization

    Cell membrane; Multi-pass membrane protein
  • Database links

    • Entrez Gene: 2859 Human
    • Omim: 602646 Human
    • SwissProt: Q9HC97 Human
    • Alternative names

      • G protein coupled receptor 35 antibody
      • G protein coupled receptor 35 antibody
      • GPR 35 antibody
      • GPR35 antibody
      • KYNA receptor antibody
      • Kynurenic acid receptor antibody
      • Probable G protein coupled receptor 35 antibody
      • Probable G protein coupled receptor GPR35 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR35 antibody (ab150635)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR35 antibody (ab150635)
      Immunohistochemical analysis of formalin fixed, paraffin embedded Human pancreas tissue labeling GPCR GPR35 with ab150635 at 23 µg/ml after heat induced antigen retrieval.
    • Immunocytochemistry - Anti-GPCR GPR35 antibody (ab150635)
      Immunocytochemistry - Anti-GPCR GPR35 antibody (ab150635)
      Immunocytochemical analysis of GPCR GPR35 transfected HEK293 Human embryonic kidney cells labeling GPCR GPR35 with ab150635.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab150635? Please let us know so that we can cite the reference in this datasheet.

    ab150635 has been referenced in 1 publication.

    • Wang W  et al. Overexpression of GPR35 confers drug resistance in NSCLC cells by ß-arrestin/Akt signaling. Onco Targets Ther 11:6249-6257 (2018). PubMed: 30288060

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GPCR GPR35 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Rat Cell (dorsal root ganglion neurons)
    Specification
    dorsal root ganglion neurons
    Permeabilization
    Yes - PBS+triton X-100 0.1%
    Fixative
    Paraformaldehyde
    Read More

    Mr. Francesco Resta

    Verified customer

    Submitted Oct 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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