Anti-GPCR GPR39 antibody - C-terminal (ab229648)

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Western blot - Anti-GPCR GPR39 antibody - C-terminal (ab229648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR39 antibody - C-terminal (ab229648)

Key features and details

  • Rabbit polyclonal to GPCR GPR39 - C-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GPCR GPR39 antibody - C-terminal
    See all GPCR GPR39 primary antibodies

  • Description

    Rabbit polyclonal to GPCR GPR39 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GPCR GPR39 (C terminal). The exact sequence is proprietary.
    Database link: O43194

  • Positive control

    • WB: U-87 MG whole cell extract. IHC-P: Human oral carcinoma tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.

Target

  • Function

    Zn(2+) acts as a agonist. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated mainly through G(q)-alpha and G(12)/G(13) proteins. Involved in regulation of body weight, gastrointestinal mobility, hormone secretion and cell death.

  • Tissue specificity

    Expressed in many tissues, including the stomach, intestine and hypothalamus.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • G protein coupled receptor GPR39 antibody
      • G Protein-Coupled Receptor 39 antibody
      • G-protein coupled receptor 39 antibody
      • GPR 39 antibody
      • Gpr39 antibody
      • GPR39_HUMAN antibody
      • MGC149541 antibody
      see all

    Images

    • Western blot - Anti-GPCR GPR39 antibody - C-terminal (ab229648)
      Western blot - Anti-GPCR GPR39 antibody - C-terminal (ab229648)
      Anti-GPCR GPR39 antibody - C-terminal (ab229648) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

      Predicted band size: 51 kDa



      10% SDS-PAGE gel.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR39 antibody - C-terminal (ab229648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR39 antibody - C-terminal (ab229648)

      Paraffin-embedded human oral carcinoma tissue stained for GPCR GPR39 using ab229648 at 1/500 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab229648? Please let us know so that we can cite the reference in this datasheet.

    ab229648 has been referenced in 1 publication.

    • Kim YJ  et al. Zn2+ stimulates salivary secretions via metabotropic zinc receptor ZnR/GPR39 in human salivary gland cells. Sci Rep 9:17648 (2019). PubMed: 31776425

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