Overview

  • Product name

  • Description

    Rabbit polyclonal to GPCR GPR40
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Recombinant fragment corresponding to Human GPCR GPR40 aa 143-178.
    Sequence:

    GLEAPGGWLDHSNTSLGINTPVNGSPVCLEAWDPAS


    Database link: O14842

  • Positive control

    • IHC-P: Human brain and pancreatic tissue. ICC/IF: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.

Target

  • Function

    Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway.
  • Tissue specificity

    Expressed abundantly in pancreatic beta cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

    • Alternative names

      • FFA1R antibody
      • FFAR 1 antibody
      • Ffar1 antibody
      • FFAR1_HUMAN antibody
      • Free fatty acid receptor 1 antibody
      • G protein coupled receptor 40 antibody
      • G-protein coupled receptor 40 antibody
      • GPCR40 antibody
      • GPR 40 antibody
      • GPR40 antibody
      see all

    Images

    • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GPCR GPR40 (green) using ab236285 at 1/133 dilution in ICC/IF. Counterstained with DAPI. The secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

       

    • Paraffin-embedded human pancreas tissue stained for GPCR GPR40 using ab236285 at 1/400 dilution in immunohistochemical analysis.

      After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

    • Paraffin-embedded human brain tissue stained for GPCR GPR40 using ab236285 at 1/400 dilution in immunohistochemical analysis.

      After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

    References

    ab236285 has not yet been referenced specifically in any publications.

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