Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 32 kDa).|
FunctionReceptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway.
Tissue specificityExpressed abundantly in pancreatic beta cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- FFA1R antibody
- FFAR 1 antibody
- Ffar1 antibody
Anti-GPCR GPR40 antibody (ab32889) at 0.03 µg/ml + Human breast lysate (35µg protein in RIPA buffer).
Predicted band size: 32 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Detected by chemiluminescence.
ab32889 has not yet been referenced specifically in any publications.