Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 32 kDa).
1 hour primary incubation is recommended for this product.
FunctionReceptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G-proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway.
Tissue specificityExpressed abundantly in pancreatic beta cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- FFA1R antibody
- FFAR 1 antibody
- Ffar1 antibody
Anti-GPCR GPR40 antibody (ab32889) at 0.03 µg/ml + Human breast lysate (35µg protein in RIPA buffer).
Predicted band size: 32 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Detected by chemiluminescence.
ab32889 has not yet been referenced specifically in any publications.