Overview

  • Product name

  • Description

    Rabbit polyclonal to GPCR GPR43
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 281-330 (RRAFGRGLQV LRNQGSSLLG RRGKDTAEGT NEDRGVGQGE GMPSSDFTTE) of Human GPCR GPR43 (NP_005297).

  • Positive control

    • Human fetal liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • Function

    Receptor for short chain fatty acids through a G(i)-protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate.
  • Tissue specificity

    Expressed at relatively high levels in peripheral blood leukocytes and, to lesser, extent, in spleen.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Fatty acid receptor 2 antibody
    • FFA2R antibody
    • Ffar2 antibody
    • FFAR2_HUMAN antibody
    • free fatty acid activated receptor 2 antibody
    • Free fatty acid receptor 2 antibody
    • G protein coupled receptor 43 antibody
    • G protein-coupled receptor 43 antibody
    • G-protein coupled receptor 43 antibody
    • GPR 43 antibody
    • GPR43 antibody
    see all

Images

  • Anti-GPCR GPR43 antibody (ab131003) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Predicted band size: 37 kDa



    Gel concentration: 12%

References

ab131003 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Kaiyuan Yang

Verified customer

Submitted Apr 28 2017

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (SDS-acrylamide 10%)
Sample
Human Cell lysate - whole cell (enteroendocrine cell)
Specification
enteroendocrine cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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