Overview

  • Product name

    Anti-GPCR GPR45 antibody - Cytoplasmic domain
  • Description

    Rabbit polyclonal to GPCR GPR45 - Cytoplasmic domain
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Monkey, Gorilla, Common marmoset, Elephant
  • Immunogen

    Synthetic peptide corresponding to 16 amino acids from the 3rd cytoplasmic domain of Human GPCR GPR45 (NP_009158.3).

  • Positive control

    • Human brain (neurons and glia) tissue; HEK293 cells transfected with GPCR GPR45.

Properties

Applications

Our Abpromise guarantee covers the use of ab150645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 42 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    GPCR GPR54 is a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPR45 is an orphan receptor, which may play a role in brain function.
  • Cellular localization

    Cell membrane; Multi-pass membrane protein
  • Database links

  • Alternative names

    • G protein coupled receptor 45 antibody
    • High affinity lysophosphatidic acid receptor antibody
    • Probable G protein coupled receptor 45 antibody
    • PSP24 1 antibody
    • PSP24 alpha antibody
    • PSP24 antibody
    • PSP24(ALPHA) antibody
    • PSP241 antibody
    • PSP24A antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue (neurons and glia), labelling GPCR GPR45 with ab150645 at 42 µg/ml.
  • Immunocytochemical analysis of HEK293 (Human embryonic kidney) cells transfected with GPCR GPR45.
  • Immunocytochemical analysis of non-transfected HEK293 (human embryonic kidney) cells.

References

ab150645 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA and Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Cell (brain)
Specification
brain
Permeabilization
Yes - Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 20 2013

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