Overview

  • Product name

    Anti-GPCR GPR48/LGR4 antibody
    See all GPCR GPR48/LGR4 primary antibodies
  • Description

    Rabbit polyclonal to GPCR GPR48/LGR4
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total GPCR GPR48/LGR4 protein.

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GPCR GPR48/LGR4 (internal sequence).

  • Positive control

    • Jurkat cell extract
  • General notes

     This product was previously labelled as GPCR GPR48

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
ELISA 1/5000.

Target

  • Function

    Orphan receptor.
  • Tissue specificity

    Expressed in multiple steroidogenic tissues: placenta, ovary, testis and adrenal. Expressed also in spinal cord, thyroid, stomach, trachea, heart, pancreas, kidney, prostate and spleen.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
    Contains 15 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BNMD17 antibody
    • G protein coupled receptor 48 antibody
    • G-protein coupled receptor 48 antibody
    • GPR48 antibody
    • Leucine-rich repeat-containing G protein-coupled receptor 4 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 4 antibody
    • LGR4 antibody
    • LGR4_HUMAN antibody
    see all

Images

  • All lanes : Anti-GPCR GPR48/LGR4 antibody (ab75501) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 104 kDa
    Observed band size: 104 kDa

References

ab75501 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your phone call. Please find the western blot protocol for use with ab75501 and ab75732 attached. For maximum signal, we recommend the overnight incubation at 4C with the primary antibody. Ab75501 should be used at a dilution between 1:500-1:1000 and ab75732 should be used at a dilution between 1:50-1:100. I hope this helps, please let me know if you need any additional information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up