Key features and details
- Rabbit polyclonal to GPCR GPR75
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GPCR GPR75 antibody
See all GPCR GPR75 primary antibodies
DescriptionRabbit polyclonal to GPCR GPR75
Specificityab75581 detects endogenous levels of total GPCR GPR75 protein.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from an internal sequence of human GPCR GPR75.
- WB: Extracts from HT-29 and K562 cells ICC/IF: LOVO cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75581 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab75581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 59 kDa.|
|ICC/IF||1/500 - 1/1000.|
Tissue specificityExpressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cell/nerve fiber layer.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- G protein coupled receptor 75 antibody
- GPR chr2 antibody
- Gpr75 antibody
All lanes : Anti-GPCR GPR75 antibody (ab75581) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells
Lane 2 : Extracts from K562 cells
Lane 3 : Extracts from HT-29 cells with Immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
ab75581, at a 1/500 dilution, staining GPCR GPR75 in LOVO cells by Immunofluorescence, in the absence (left image) and presence (right image) of the immunising peptide.
ab75581 has not yet been referenced specifically in any publications.