Overview

  • Product name

  • Description

    Rabbit polyclonal to GPCR GPR75
  • Host species

    Rabbit
  • Specificity

    ab75581 detects endogenous levels of total GPCR GPR75 protein.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human GPCR GPR75.

  • Positive control

    • WB: Extracts from HT-29 and K562 cells ICC/IF: LOVO cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab75581 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Orphan receptor.
  • Tissue specificity

    Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cell/nerve fiber layer.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • G protein coupled receptor 75 antibody
    • GPR chr2 antibody
    • Gpr75 antibody
    • GPR75_HUMAN antibody
    • GPRchr2 antibody
    • OTTHUMP00000159608 antibody
    • Probable G protein coupled receptor 75 antibody
    • Probable G-protein coupled receptor 75 antibody
    • WI 31133 antibody
    • WI31133 antibody
    see all

Images

  • All lanes : Anti-GPCR GPR75 antibody (ab75581) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from K562 cells
    Lane 3 : Extracts from HT-29 cells with Immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • ab75581, at a 1/500 dilution, staining GPCR GPR75 in LOVO cells by Immunofluorescence, in the absence (left image) and presence (right image) of the immunising peptide.

References

ab75581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

I have been able to contact the lab regarding your question about ab75581.This antibody binds to the cytoplasmic domain of target protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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