Key features and details
- Goat polyclonal to GPCR GPR81
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab106942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product.
FunctionActs as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.
Tissue specificityExpressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
FormG protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
- FKSG80 antibody
- FKSG80 protein antibody
- G protein coupled receptor 104 antibody
Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/ml + Human liver lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab106942 has been referenced in 1 publication.
- Wagner W et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). WB ; Human . PubMed: 26208712