Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10191] to GPCR GPRC5B
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-GPCR GPRC5B antibody [EPR10191]
See all GPCR GPRC5B primary antibodies
DescriptionRabbit monoclonal [EPR10191] to GPCR GPRC5B
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GPCR GPRC5B aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NZH0
- Human fetal kidney, HUVEC, 293T, Jurkat and Human pancreas lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab176326 in the following tested applications.
|WB||1/10000 - 1/50000. Predicted molecular weight: 45 kDa.|
|IP||1/10 - 1/100.|
FunctionUnknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Tissue specificityExpression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to PubMed:10945465, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal chord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane. Cytoplasmic vesicle membrane. Localized in the plasma membrane and perinuclear vesicles.
- Information by UniProt
- A 69G12.1 antibody
- A-69G12.1 antibody
- G protein coupled receptor family C group 1 member B antibody
All lanes : Anti-GPCR GPRC5B antibody [EPR10191] (ab176326) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : HUVEC cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Human pancreas lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
Western blot analysis on immunoprecipitation pellet from (Lane 1) Jurkat cells lysate or (Lane 2) 1XPBS (negative control) using ab176326 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab176326 has not yet been referenced specifically in any publications.