Overview

  • Product name

    Anti-GPCR LGR6 antibody [EPR6874]
    See all GPCR LGR6 primary antibodies
  • Description

    Rabbit monoclonal [EPR6874] to GPCR LGR6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GPCR LGR6 aa 850-950 (intracellular). The exact sequence is proprietary.
    Database link: Q9HBX8

  • Positive control

    • Human uterus, ovary, HUVEC, Human adrenal gland and HeLa lysates; Human adrenal gland tissue; HUVEC cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6874
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution

      Lane 1 : Human uterus lysate
      Lane 2 : Human ovary lysate
      Lane 3 : HUVEC lysate
      Lane 4 : Human adrenal gland lysate
      Lane 5 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 104 kDa

    • Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution + GST-Tagged Recombinant Human GPCR LGR6 protein (aa 1 to 828) at 0.015 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 104 kDa


      Exposure time: 10 seconds


      Recombinant Human GPCR LGR6 protein (Cat#:  ab153472)

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling GPCR LGR6 with purified ab126747 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Overlay histogram showing LoVo cells stained with ab126747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126747, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:

    • Shi J  et al. Human nail stem cells are retained but hypofunctional during aging. J Mol Histol 49:303-316 (2018). IHC-Fr . Read more (PubMed: 29633149) »
    • Wu V  et al. ALDH1A1 positive cells are a unique component of the tonsillar crypt niche and are lost along with NGFR positive stem cells during tumourigenesis. Pathology N/A:N/A (2018). WB . Read more (PubMed: 29891189) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Stomach)
    Permeabilization
    No
    Specification
    Stomach
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Mar 27 2019

    Question
    Answer

    Thank you for your reply.

    You could make an antibody against Zebrafish lgr6 but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:




    I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

    Read More

    Answer

    Thank you for supplying that information.

    I aligned the zebrafish sequence with the human sequence and there was only a 59% level of homology, between the 2. I also aligned the actual immunogen sequence used for ab126747, and the level of homology for that particular sequence was about 50%.

    Based on this information, I do not think that any of the lgr6 antibodies that we have will react with Zebrafish.

    If there is anything else I can help you with, please let me know.

    Read More

    Answer

    Thank you for contacting Abcam.

    I would be happy to help determine the level of homolgoy between our lgr6 antibodies and Zebrafish. However I am unable to locate the Zebrafish lgr6 sequence, and so I was wandering if you would be able to provide that sequence so that I can perform the alignment.

    I look forward to your reply and helping you with your enquiry.

    Read More

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