RecombinantRabMAb

Recombinant Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

Reviews (1)Q&A (3)References (5)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6874] to GPCR LGR6
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GPCR LGR6 antibody [EPR6874]
    See all GPCR LGR6 primary antibodies

  • Description

    Rabbit monoclonal [EPR6874] to GPCR LGR6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GPCR LGR6 aa 850-950 (intracellular). The exact sequence is proprietary.
    Database link: Q9HBX8

  • Positive control

    • Human uterus, ovary, HUVEC, Human adrenal gland and HeLa lysates; Human adrenal gland tissue; HUVEC cells IHC: Human lung tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6874

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution

      Lane 1 : Human uterus lysate
      Lane 2 : Human ovary lysate
      Lane 3 : HUVEC lysate
      Lane 4 : Human adrenal gland lysate
      Lane 5 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 104 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling GPCR LGR6 with ab126747 at 1/250 dilution (4.4 μg/mL), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Positive staining on human lung carcinoma. The section was incubated with ab126747 for 30 minutes at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunocytochemistry/ Immunofluorescence - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Immunocytochemistry/ Immunofluorescence - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling GPCR LGR6 with purified ab126747 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution + GST-Tagged Recombinant Human GPCR LGR6 protein (aa 1 to 828) at 0.015 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 104 kDa


      Exposure time: 10 seconds


      Recombinant Human GPCR LGR6 protein (Cat#:  ab153472)

    • Flow Cytometry - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Flow Cytometry - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
      Overlay histogram showing LoVo cells stained with ab126747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126747, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References (5)

    Publishing research using ab126747? Please let us know so that we can cite the reference in this datasheet.

    ab126747 has been referenced in 5 publications.

    • Chen H  et al. Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells. Sci Adv 6:eaay1514 (2020). PubMed: 32284993
    • Munguía-Reyes A  et al. R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior. Am J Respir Cell Mol Biol 59:65-76 (2018). PubMed: 29345973
    • Shi J  et al. Human nail stem cells are retained but hypofunctional during aging. J Mol Histol 49:303-316 (2018). IHC-Fr . PubMed: 29633149
    • Wu V  et al. ALDH1A1 positive cells are a unique component of the tonsillar crypt niche and are lost along with NGFR positive stem cells during tumourigenesis. Pathology N/A:N/A (2018). WB . PubMed: 29891189
    • Ahmed NS  et al. Epidermal E-Cadherin Dependent ß-Catenin Pathway Is Phytochemical Inducible and Accelerates Anagen Hair Cycling. Mol Ther 25:2502-2512 (2017). PubMed: 28803863

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GPCR LGR6 antibody [EPR6874]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Stomach)
    Permeabilization
    No
    Specification
    Stomach
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 27 2019

    Question

    Can a lgr6 antibody be made against zebrafish protein.

    Read More
    Answer

    Thank you for your reply.

    You could make an antibody against Zebrafish lgr6 but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:




    I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

    Read More

    Question

    Thank you for the mail and helping me out in the homology search, I am pasting the accession number of the gene

    Read More
    Answer

    Thank you for supplying that information.

    I aligned the zebrafish sequence with the human sequence and there was only a 59% level of homology, between the 2. I also aligned the actual immunogen sequence used for ab126747, and the level of homology for that particular sequence was about 50%.

    Based on this information, I do not think that any of the lgr6 antibodies that we have will react with Zebrafish.

    If there is anything else I can help you with, please let me know.

    Read More

    Question

    WE are going to order lgr6 antibody, there are three anitobodies listed in your catalogue, we are going to use this antibody in zebrafish, can you please check for the homology match of the zebrafish lgr6 protein and the peptide against which the antibody is raised, so that we can decide which antibody to order

    Read More
    Answer

    Thank you for contacting Abcam.

    I would be happy to help determine the level of homolgoy between our lgr6 antibodies and Zebrafish. However I am unable to locate the Zebrafish lgr6 sequence, and so I was wandering if you would be able to provide that sequence so that I can perform the alignment.

    I look forward to your reply and helping you with your enquiry.

    Read More

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