Product nameAnti-GPCR LGR6 antibody [EPR6874]
See all GPCR LGR6 primary antibodies
DescriptionRabbit monoclonal [EPR6874] to GPCR LGR6
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GPCR LGR6 aa 850-950 (intracellular). The exact sequence is proprietary.
Database link: Q9HBX8
- Human uterus, ovary, HUVEC, Human adrenal gland and HeLa lysates; Human adrenal gland tissue; HUVEC cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 104 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/500.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Contains 15 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell membrane.
- Information by UniProt
- FLJ14471 antibody
- Gonadotropin receptor antibody
- GPCR antibody
All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution
Lane 1 : Human uterus lysate
Lane 2 : Human ovary lysate
Lane 3 : HUVEC lysate
Lane 4 : Human adrenal gland lysate
Lane 5 : HeLa lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 104 kDa
Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution + GST-Tagged Recombinant Human GPCR LGR6 protein (aa 1 to 828) at 0.015 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 104 kDa
Exposure time: 10 seconds
Recombinant Human GPCR LGR6 protein (Cat#: ab153472)
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling GPCR LGR6 with purified ab126747 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Overlay histogram showing LoVo cells stained with ab126747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126747, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Shi J et al. Human nail stem cells are retained but hypofunctional during aging. J Mol Histol 49:303-316 (2018). IHC-Fr . Read more (PubMed: 29633149) »
- Wu V et al. ALDH1A1 positive cells are a unique component of the tonsillar crypt niche and are lost along with NGFR positive stem cells during tumourigenesis. Pathology N/A:N/A (2018). WB . Read more (PubMed: 29891189) »