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RecombinantRabMAb

Recombinant Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

Reviews (1)Q&A (3)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Immunocytochemistry - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Flow Cytometry (Intracellular) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
  • Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6874] to GPCR LGR6
  • Suitable for: Flow Cyt (Intra), ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

Overview

  • Product name

    Anti-GPCR LGR6 antibody [EPR6874]
    See all GPCR LGR6 primary antibodies

  • Description

    Rabbit monoclonal [EPR6874] to GPCR LGR6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat and mouse liver tissues. Human lung tissue; WB: HEK-293, HeLa, HUVEC, and SK-OV-3 cell lysates. Mouse, rat, and human liver tissue lysates. Mouse and rat lung tissue lysates; ICC and Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6874

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
1/50.

For unpurified use at 1/100 - 1/500.
WB
1/1000. Predicted molecular weight: 104 kDa.

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/240. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/250.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
1/50.

For unpurified use at 1/100 - 1/500.
WB
1/1000. Predicted molecular weight: 104 kDa.

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/240. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/250.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling GPCR LGR6 with purified ab126747 at 1/240 dilution (0.61 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    All lanes : Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
    Lane 4 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
    Lane 5 : Mouse liver lysate
    Lane 6 : Rat liver lysate
    Lane 7 : Human liver lysate
    Lane 8 : Mouse lung lysate
    Lane 9 : Rat lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 104 kDa



    We are not sure about the extra band.

  • Immunocytochemistry - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunocytochemistry - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GPCR LGR6 with Purified ab126747 at 1:50 dilution (3.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry (Intracellular) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Flow Cytometry (Intracellular) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GPCR LGR6 with Purified ab126747 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling GPCR LGR6 with purified ab126747 at 1/240 dilution (0.61 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling GPCR LGR6 with purified ab126747 at 1/240 dilution (0.61 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Western blot - Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Anti-GPCR LGR6 antibody [EPR6874] (ab126747) at 1/1000 dilution + GST-Tagged Recombinant Human GPCR LGR6 protein at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 104 kDa


    Exposure time: 10 seconds


    Recombinant Human GPCR LGR6 protein (Cat#:  ab153472)

  • Anti-GPCR LGR6 antibody [EPR6874] (ab126747)
    Anti-GPCR LGR6 antibody [EPR6874] (ab126747)

References (8)

Publishing research using ab126747? Please let us know so that we can cite the reference in this datasheet.

ab126747 has been referenced in 8 publications.

  • Chen H  et al. Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells. Sci Adv 6:eaay1514 (2020). PubMed: 32284993
  • Kim DE  et al. Deficiency in the DNA repair protein ERCC1 triggers a link between senescence and apoptosis in human fibroblasts and mouse skin. Aging Cell 19:e13072 (2020). PubMed: 31737985
  • Jank BJ  et al. Epithelial stem cell marker LGR6 expression identifies a low-risk subgroup in human papillomavirus positive oropharyngeal squamous cell carcinoma. Oral Oncol 105:104657 (2020). PubMed: 32244172
  • Li B  et al. Skin Epidermis and Adnexa Regrowth Induced by Treatment With Artificial Dermal Template After Full-Thickness Skin Wound. Int J Low Extrem Wounds 18:42-55 (2019). PubMed: 30616449
  • Shi J  et al. Human nail stem cells are retained but hypofunctional during aging. J Mol Histol 49:303-316 (2018). IHC-Fr . PubMed: 29633149
  • Wu V  et al. ALDH1A1 positive cells are a unique component of the tonsillar crypt niche and are lost along with NGFR positive stem cells during tumourigenesis. Pathology N/A:N/A (2018). WB . PubMed: 29891189
  • Munguía-Reyes A  et al. R-Spondin-2 Is Upregulated in Idiopathic Pulmonary Fibrosis and Affects Fibroblast Behavior. Am J Respir Cell Mol Biol 59:65-76 (2018). PubMed: 29345973
  • Ahmed NS  et al. Epidermal E-Cadherin Dependent ß-Catenin Pathway Is Phytochemical Inducible and Accelerates Anagen Hair Cycling. Mol Ther 25:2502-2512 (2017). PubMed: 28803863

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-GPCR LGR6 antibody [EPR6874]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Stomach)
Permeabilization
No
Specification
Stomach
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Mar 27 2019

Question

Can a lgr6 antibody be made against zebrafish protein.

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Answer

Thank you for your reply.

You could make an antibody against Zebrafish lgr6 but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company. For more information about these services visit:




I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

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Question

Thank you for the mail and helping me out in the homology search, I am pasting the accession number of the gene

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Answer

Thank you for supplying that information.

I aligned the zebrafish sequence with the human sequence and there was only a 59% level of homology, between the 2. I also aligned the actual immunogen sequence used for ab126747, and the level of homology for that particular sequence was about 50%.

Based on this information, I do not think that any of the lgr6 antibodies that we have will react with Zebrafish.

If there is anything else I can help you with, please let me know.

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Question

WE are going to order lgr6 antibody, there are three anitobodies listed in your catalogue, we are going to use this antibody in zebrafish, can you please check for the homology match of the zebrafish lgr6 protein and the peptide against which the antibody is raised, so that we can decide which antibody to order

Read More
Answer

Thank you for contacting Abcam.

I would be happy to help determine the level of homolgoy between our lgr6 antibodies and Zebrafish. However I am unable to locate the Zebrafish lgr6 sequence, and so I was wandering if you would be able to provide that sequence so that I can perform the alignment.

I look forward to your reply and helping you with your enquiry.

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