Key features and details
- Rabbit polyclonal to GPCR MRGX2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GPCR MRGX2 antibody
See all GPCR MRGX2 primary antibodies
DescriptionRabbit polyclonal to GPCR MRGX2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: HEK-293T and MCF7 whole cell lysate; Mouse liver tissue lysate. ICC/IF: MCF7 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|ICC/IF||1/50 - 1/200.|
FunctionOrphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled.
Tissue specificityHas a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Mas subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- G protein coupled receptor MRGX 2 antibody
- G protein coupled receptor MRGX2 antibody
- Mas related G protein coupled receptor member X2 antibody
MCF7 (Human breast adenocarcinoma cell line) cells stained for GPCR MRGX2 (Green) using ab237047 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
All lanes : Anti-GPCR MRGX2 antibody (ab237047) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237047 has not yet been referenced specifically in any publications.