Overview

  • Product name

    Anti-GPCR RDC1/CXCR-7 antibody
    See all GPCR RDC1/CXCR-7 primary antibodies
  • Description

    Rabbit polyclonal to GPCR RDC1/CXCR-7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab38088)

  • Positive control

    • This antibody gave a positive result with human brain, testis and heart tissue lysates. Human kidney is a suitable positive control for IHC.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 52 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human GPCR RDC1/CXCR-7 peptide (ab38088).

GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.

IHC-P 1/100.

Target

  • Function

    Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
  • Tissue specificity

    Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACKR3 antibody
    • atypical chemokine receptor 3 antibody
    • C X C chemokine receptor type 7 antibody
    • C-X-C chemokine receptor type 7 antibody
    • Chemokine (C-X-C motif) receptor 7 antibody
    • Chemokine C X C motif receptor 7 antibody
    • Chemokine orphan receptor 1 antibody
    • CMKOR1 antibody
    • CXC R7 antibody
    • CXC-R7 antibody
    • CXCR 7 antibody
    • CXCR-7 antibody
    • CXCR7 antibody
    • CXCR7_HUMAN antibody
    • G protein coupled receptor 159 antibody
    • G protein coupled receptor antibody
    • G protein coupled receptor RDC1 homolog antibody
    • G-protein coupled receptor 159 antibody
    • G-protein coupled receptor RDC1 homolog antibody
    • GPCR RDC1 antibody
    • GPR159 antibody
    • GPRN1 antibody
    • RDC 1 antibody
    • RDC-1 antibody
    • RDC1 antibody
    see all

Images

  • All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab38089) at 1/250 dilution

    Lane 1 : Human Brain Tissue Lysate
    Lane 2 : Human Testis Tissue Lysate
    Lane 3 : Human Liver Tissue Lysate
    Lane 4 : Human Heart Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
  • ab38089 staining pancreas (human and mouse) kidney (mouse and rat) and testis (rat and human) tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Zhang L  et al. Cytokine augments the sorafenib-induced apoptosis in Huh7 liver cancer cell by inducing mitochondrial fragmentation and activating MAPK-JNK signalling pathway. Biomed Pharmacother 110:213-223 (2019). Read more (PubMed: 30476722) »
  • Küçükköse C & Yalçin Özuysal Ö Effects of Notch signalling on the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20 in breast cancer. Turk J Biol 43:70-76 (2019). Read more (PubMed: 30930637) »
See all 20 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 23 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, Jurkat)
Loading amount
100 µg
Specification
HeLa, Jurkat
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C

Mrs. Merly Saare

Verified customer

Submitted Sep 22 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas (human) kidney and pancreas (mouse) kidne)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate Buffer
Permeabilization
No
Specification
Pancreas (human) kidney and pancreas (mouse) kidne
Blocking step
sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Mr. Antibody Solutions

Verified customer

Submitted Apr 18 2008

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