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    gpcr-rdc1cxcr-7-antibody-ab72100.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (27)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
  • Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
  • Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

Key features and details

  • Rabbit polyclonal to GPCR RDC1/CXCR-7
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
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Recombinant Human GPCR RDC1/CXCR-7 protein (ab153435)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GPCR RDC1/CXCR-7 antibody
    See all GPCR RDC1/CXCR-7 primary antibodies
  • Description

    Rabbit polyclonal to GPCR RDC1/CXCR-7
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 106-117.
    Sequence:

    QHNQWPMGELTC

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant Human GPCR RDC1/CXCR-7 protein (ab153435)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Chicken
Xenopus laevis
Application Abreviews Notes
WB
Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
Use a concentration of 10 µg/ml.

Target

  • Function

    Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
  • Tissue specificity

    Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P25106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57007 Human
    • Entrez Gene: 12778 Mouse
    • Omim: 610376 Human
    • SwissProt: P25106 Human
    • SwissProt: P56485 Mouse
    • Unigene: 471751 Human
    • Unigene: 6522 Mouse
    • Alternative names

      • ACKR3 antibody
      • atypical chemokine receptor 3 antibody
      • C X C chemokine receptor type 7 antibody
      • C-X-C chemokine receptor type 7 antibody
      • Chemokine (C-X-C motif) receptor 7 antibody
      • Chemokine C X C motif receptor 7 antibody
      • Chemokine orphan receptor 1 antibody
      • CMKOR1 antibody
      • CXC R7 antibody
      • CXC-R7 antibody
      • CXCR 7 antibody
      • CXCR-7 antibody
      • CXCR7 antibody
      • CXCR7_HUMAN antibody
      • G protein coupled receptor 159 antibody
      • G protein coupled receptor antibody
      • G protein coupled receptor RDC1 homolog antibody
      • G-protein coupled receptor 159 antibody
      • G-protein coupled receptor RDC1 homolog antibody
      • GPCR RDC1 antibody
      • GPR159 antibody
      • GPRN1 antibody
      • RDC 1 antibody
      • RDC-1 antibody
      • RDC1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

      Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml

    • Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 5 µg/ml

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate with immunizing peptide

      Predicted band size: 42 kDa
      Observed band size: 42 kDa
      Additional bands at: 29 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 2 µg/ml

      Lane 1 : Human spleen with 5% non-fat milk in TBST
      Lane 2 : Mouse spleen with 5% non-fat milk in TBST
      Lane 3 : Human tonsil with 5% non-fat milk in TBST
      Lane 4 : Human lymph node with 5% non-fat milk in TBST

      Secondary
      All lanes : anti-rabbit HRP

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

      IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (27)

    Publishing research using ab72100? Please let us know so that we can cite the reference in this datasheet.

    ab72100 has been referenced in 27 publications.

    • Li T  et al. Microglia induce the transformation of A1/A2 reactive astrocytes via the CXCR7/PI3K/Akt pathway in chronic post-surgical pain. J Neuroinflammation 17:211 (2020). PubMed: 32665021
    • Yu G  et al. Sitagliptin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in Aneurysm Necks Through the SDF-1/CXCR4/NRF2 Signaling Pathway. Front Endocrinol (Lausanne) 10:823 (2019). PubMed: 32038475
    • Macoska JA  et al. Inhibition of the CXCL12/CXCR4 axis prevents periurethral collagen accumulation and lower urinary tract dysfunction in vivo. Prostate 79:757-767 (2019). PubMed: 30811623
    • Xiang DN  et al. Platelet-derived growth factor-BB promotes proliferation and migration of retinal microvascular pericytes by up-regulating the expression of C-X-C chemokine receptor types 4. Exp Ther Med 18:4022-4030 (2019). PubMed: 31611940
    • McGuinness D  et al. A molecular signature for delayed graft function. Aging Cell 17:e12825 (2018). PubMed: 30094915
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Hi!!

    Yes, please request a certificate of quality



    GPCR RDC1 100UG

    Bacth: GR62219-1

    Catalog: AB72100

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2012

    Answer

    Thank you for your enquiry.

    I have attached the requested Certificate of Compliance.

    Please let me know if you need anything further.

    Read More

    Abcam Scientific Support

    Answered on Apr 23 2012

    Question

    Hi,

    Trusted certificate request



    GPCR RDC1 100UG

    Bacth: GR62219-1

    Catalog: AB72100

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2012

    Answer

    Thanks for your email.

    I can generatea Certificate of Compliance for ab72100. Is this acceptable?

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2012

    Question

    Hi there,
    We would like to purchase a CXCR4 CXCR7 CXCL12 antibody for IHC on paraffin
    tissues.
    Which one would you reccomend? do you guarantee that they work?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2012

    Answer

    Thank you for contacting us.

    I am happy to let you know that we have the following antibodies available that we can guarantee to work in IHC on paraffin-embedded tissue with human samples:

    CXCR4
    Rabbit polyclonal ab2074:
    Click here (or use the following: https://www.abcam.com/CXCR4-antibody-ab2074.html).

    Goat polyclonal ab1671:
    Click here (or use the following: https://www.abcam.com/CXCR4-antibody-ab1671.html).

    Mouse monoclonal ab58176:
    Click here (or use the following: https://www.abcam.com/CXCR4-antibody-ab58176.html).

    CXCR7
    Rabbit polyclonal ab72100:
    Click here (or use the following: https://www.abcam.com/GPCR-RDC1CXCR-7-antibody-ab72100.html).

    CXCL12 (SDF1 alpha)
    Rabbit polyclonal ab9797:
    Click here (or use the following: https://www.abcam.com/SDF1-antibody-ab9797.html).

    Mouse monoclonal ab89321:
    Click here (or use the following: https://www.abcam.com/SDF1-antibody-MM0211-9N26-ab89321.html).

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

    Please note that ab89321 qualifies for our current discount programme: We do not currently have an image of ab89321 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab89321 now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

    It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab89321 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab89321 so please wait for my reply before ordering.

    2. Purchase ab89321 either by phone, fax, or online (www.abcam.com).

    3. Use it in IHC-P.

    4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated!

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 28 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-GPCR RDC1/CXCR-7 antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain tissue)
    Permeabilization
    No
    Specification
    Brain tissue
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
    Fixative
    Acetone/ Methanol 1:1
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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