- Datasheet
- Specific References (20)
- Protocols
Overview
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Product nameAnti-GPCR RDC1/CXCR-7 antibody
See all GPCR RDC1/CXCR-7 primary antibodies -
DescriptionRabbit polyclonal to GPCR RDC1/CXCR-7
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Horse, Cow, Human, Chimpanzee, Monkey
Predicted to work with: Chicken, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 106-117.
Sequence:QHNQWPMGELTC
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Positive control
- Jurkat cell lysate; human tonsil tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab72100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 10 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| WB | Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 22745793 |
Target
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FunctionReceptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
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Tissue specificityExpressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 509585 Cow
- Entrez Gene: 100057501 Horse
- Entrez Gene: 57007 Human
- Entrez Gene: 12778 Mouse
- Entrez Gene: 84348 Rat
- Omim: 610376 Human
- SwissProt: P25106 Human
- SwissProt: P56485 Mouse
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Alternative names
- ACKR3 antibody
- atypical chemokine receptor 3 antibody
- C X C chemokine receptor type 7 antibody
see all
Images
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Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with immunizing peptide
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 29 kDa, 65 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml
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Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 2 µg/ml
Lane 1 : Human spleen with 5% non-fat milk in TBST
Lane 2 : Mouse spleen with 5% non-fat milk in TBST
Lane 3 : Human tonsil with 5% non-fat milk in TBST
Lane 4 : Human lymph node with 5% non-fat milk in TBST
Secondary
All lanes : anti-rabbit HRP
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)Image from Tomchuck SL et al., PLoS One. 2012;7(6):e39592. Epub 2012 Jun 22. Fig 4.; doi:10.1371/journal.pone.0039592; June 22, 2012, PLoS ONE 7(6): e39592.Immunofluorescence analysis of Human bone marrow-derived Multipotent Stromal Cells staining GPCR RDC1/CXCR-7 (green) with ab72100.
Human MSC were treated with poly(I:C) for 6 hours before staining with primary antibody and an AlexaFluor®488-conjugated secondary antibody. Nuclei were stained with DAPI (blue).
Protocols
References
This product has been referenced in:
- Zheng J et al. Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7. Reprod Biol Endocrinol 16:17 (2018). Read more (PubMed: 29499763) »
- Yang LX et al. Improvement of therapeutic effects of mesenchymal stem cells in myocardial infarction through genetic suppression of microRNA-142. Oncotarget 8:85549-85558 (2017). Read more (PubMed: 29156740) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"