Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

Reviews (1)Q&A (3)References (24)

Overview

  • Product name
    Anti-GPCR RDC1/CXCR-7 antibody
    See all GPCR RDC1/CXCR-7 primary antibodies
  • Description
    Rabbit polyclonal to GPCR RDC1/CXCR-7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Cow, Human, Chimpanzee, Monkey
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 106-117.
    Sequence:

    QHNQWPMGELTC

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat whole cell lysate (ab7899); human tonsil tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22745793

Target

  • Function
    Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
  • Tissue specificity
    Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ACKR3 antibody
    • atypical chemokine receptor 3 antibody
    • C X C chemokine receptor type 7 antibody
    • C-X-C chemokine receptor type 7 antibody
    • Chemokine (C-X-C motif) receptor 7 antibody
    • Chemokine C X C motif receptor 7 antibody
    • Chemokine orphan receptor 1 antibody
    • CMKOR1 antibody
    • CXC R7 antibody
    • CXC-R7 antibody
    • CXCR 7 antibody
    • CXCR-7 antibody
    • CXCR7 antibody
    • CXCR7_HUMAN antibody
    • G protein coupled receptor 159 antibody
    • G protein coupled receptor antibody
    • G protein coupled receptor RDC1 homolog antibody
    • G-protein coupled receptor 159 antibody
    • G-protein coupled receptor RDC1 homolog antibody
    • GPCR RDC1 antibody
    • GPR159 antibody
    • GPRN1 antibody
    • RDC 1 antibody
    • RDC-1 antibody
    • RDC1 antibody
    see all

Images

  • Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with immunizing peptide

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 29 kDa, 65 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

    Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml

  • Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    Western blot - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 2 µg/ml

    Lane 1 : Human spleen with 5% non-fat milk in TBST
    Lane 2 : Mouse spleen with 5% non-fat milk in TBST
    Lane 3 : Human tonsil with 5% non-fat milk in TBST
    Lane 4 : Human lymph node with 5% non-fat milk in TBST

    Secondary
    All lanes : anti-rabbit HRP

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)

    IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)
    Immunocytochemistry/ Immunofluorescence - Anti-GPCR RDC1/CXCR-7 antibody (ab72100)Image from Tomchuck SL et al., PLoS One. 2012;7(6):e39592. Epub 2012 Jun 22. Fig 4.; doi:10.1371/journal.pone.0039592; June 22, 2012, PLoS ONE 7(6): e39592.

    Immunofluorescence analysis of Human bone marrow-derived Multipotent Stromal Cells staining GPCR RDC1/CXCR-7 (green) with ab72100.

    Human MSC were treated with poly(I:C) for 6 hours before staining with primary antibody and an AlexaFluor®488-conjugated secondary antibody. Nuclei were stained with DAPI (blue).

References

This product has been referenced in:
  • Macoska JA  et al. Inhibition of the CXCL12/CXCR4 axis prevents periurethral collagen accumulation and lower urinary tract dysfunction in vivo. Prostate 79:757-767 (2019). Read more (PubMed: 30811623) »
  • McGuinness D  et al. A molecular signature for delayed graft function. Aging Cell 17:e12825 (2018). Read more (PubMed: 30094915) »
See all 24 Publications for this product

Publishing research using ab72100? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Hi!!

Yes, please request a certificate of quality



GPCR RDC1 100UG

Bacth: GR62219-1

Catalog: AB72100

Read More
Answer

Thank you for your enquiry.

I have attached the requested Certificate of Compliance.

Please let me know if you need anything further.

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Question

Hi,

Trusted certificate request



GPCR RDC1 100UG

Bacth: GR62219-1

Catalog: AB72100

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Answer

Thanks for your email.

I can generatea Certificate of Compliance for ab72100. Is this acceptable?

Read More

Question

Hi there,
We would like to purchase a CXCR4 CXCR7 CXCL12 antibody for IHC on paraffin
tissues.
Which one would you reccomend? do you guarantee that they work?

Read More
Answer

Thank you for contacting us.

I am happy to let you know that we have the following antibodies available that we can guarantee to work in IHC on paraffin-embedded tissue with human samples:

CXCR4
Rabbit polyclonal ab2074:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=2074).

Goat polyclonal ab1671:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=1671).

Mouse monoclonal ab58176:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=58176).

CXCR7
Rabbit polyclonal ab72100:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=72100).

CXCL12 (SDF1 alpha)
Rabbit polyclonal ab9797:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=9797).

Mouse monoclonal ab89321:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=89321).

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

Please note that ab89321 qualifies for our current discount programme: We do not currently have an image of ab89321 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab89321 now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab89321 in IHC-P. I will then send a discount code. This code must be issued before purchasing ab89321 so please wait for my reply before ordering.

2. Purchase ab89321 either by phone, fax, or online (www.abcam.com).

3. Use it in IHC-P.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Immunohistochemistry (Frozen sections) abreview for Anti-GPCR RDC1/CXCR-7 antibody

 Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain tissue)
Permeabilization
No
Specification
Brain tissue
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Acetone/ Methanol 1:1
Read More

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