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Our Abpromise guarantee covers the use of ab72100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 10 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22745793|
Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Immunofluorescence analysis of Human bone marrow-derived Multipotent Stromal Cells staining GPCR RDC1/CXCR-7 (green) with ab72100.
Human MSC were treated with poly(I:C) for 6 hours before staining with primary antibody and an AlexaFluor®488-conjugated secondary antibody. Nuclei were stained with DAPI (blue).
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