Product nameAnti-GPCR RDC1/CXCR-7 antibody
See all GPCR RDC1/CXCR-7 primary antibodies
DescriptionRabbit polyclonal to GPCR RDC1/CXCR-7
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Horse, Cow, Human, Chimpanzee, Monkey
Predicted to work with: Chicken, Xenopus laevis
- Jurkat whole cell lysate (ab7899); human tonsil tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab72100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 10 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22745793|
FunctionReceptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
Tissue specificityExpressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- ACKR3 antibody
- atypical chemokine receptor 3 antibody
- C X C chemokine receptor type 7 antibody
All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with immunizing peptide
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 29 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of GPCR RDC1/CXCR-7 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml
All lanes : Anti-GPCR RDC1/CXCR-7 antibody (ab72100) at 2 µg/ml
Lane 1 : Human spleen with 5% non-fat milk in TBST
Lane 2 : Mouse spleen with 5% non-fat milk in TBST
Lane 3 : Human tonsil with 5% non-fat milk in TBST
Lane 4 : Human lymph node with 5% non-fat milk in TBST
All lanes : anti-rabbit HRP
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil staining GPCR RDC1/CXCR-7 with ab72100 (5ug/ml). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 5ug/ml concentration ofab72100. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Immunofluorescence analysis of Human bone marrow-derived Multipotent Stromal Cells staining GPCR RDC1/CXCR-7 (green) with ab72100.
Human MSC were treated with poly(I:C) for 6 hours before staining with primary antibody and an AlexaFluor®488-conjugated secondary antibody. Nuclei were stained with DAPI (blue).
This product has been referenced in:
- Zheng J et al. Modulatory effects of trophoblast-secreted CXCL12 on the migration and invasion of human first-trimester decidual epithelial cells are mediated by CXCR4 rather than CXCR7. Reprod Biol Endocrinol 16:17 (2018). Read more (PubMed: 29499763) »
- Yang LX et al. Improvement of therapeutic effects of mesenchymal stem cells in myocardial infarction through genetic suppression of microRNA-142. Oncotarget 8:85549-85558 (2017). Read more (PubMed: 29156740) »