Recombinant Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9321] to GPCR RDC1/CXCR-7
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GPCR RDC1/CXCR-7 antibody [EPR9321]
See all GPCR RDC1/CXCR-7 primary antibodies -
Description
Rabbit monoclonal [EPR9321] to GPCR RDC1/CXCR-7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 300-400 (C terminal).
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Positive control
- THP-1, K562, Raji, C6, Raw 264.7, and NIH3T3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9321 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. -
Tissue specificity
Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57007 Human
- Entrez Gene: 12778 Mouse
- Entrez Gene: 84348 Rat
- Omim: 610376 Human
- SwissProt: P25106 Human
- SwissProt: P56485 Mouse
- SwissProt: O89039 Rat
- Unigene: 471751 Human
see all -
Alternative names
- ACKR3 antibody
- atypical chemokine receptor 3 antibody
- C X C chemokine receptor type 7 antibody
see all
Images
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All lanes : Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution (purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution (purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
All lanes : Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/1000 dilution (unpurified)
Lane 1 : THP-1 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab138509 has been referenced in 10 publications.
- Jiang C et al. Upregulating CXCR7 accelerates endothelial progenitor cell-mediated endothelial repair by activating Akt/Keap-1/Nrf2 signaling in diabetes mellitus. Stem Cell Res Ther 12:264 (2021). PubMed: 33941256
- Guo CH et al. Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice. Mar Drugs 19:N/A (2021). PubMed: 33805447
- Luo Y et al. Enzalutamide-Resistant Progression of Castration-Resistant Prostate Cancer Is Driven via the JAK2/STAT1-Dependent Pathway. Front Mol Biosci 8:652443 (2021). PubMed: 34746227
- Yu L et al. Modulation of CXC-motif chemokine receptor 7, but not 4, expression is related to migration of the human prostate cancer cell LNCaP: regulation by androgen and inflammatory stimuli. Inflamm Res 69:167-178 (2020). PubMed: 31865399
- Liu H et al. Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cells. Respir Res 21:287 (2020). PubMed: 33129326
- Ou X et al. Forkhead box P3 gene silencing inhibits the expression of chemokines and chemokine receptors associated with cell growth, migration, and apoptosis in hepatocellular carcinoma cells. Exp Ther Med 18:1091-1098 (2019). PubMed: 31316604
- Yu L et al. Transcriptional and translational-uncoupling in regulation of the CXCL12 and its receptors CXCR4, 7 in THP-1 monocytes and macrophages. Immun Inflamm Dis 6:106-116 (2018). PubMed: 29105376
- Puchert M et al. The 5T4 oncofetal glycoprotein does not act as a general organizer of the CXCL12 system in cancer cells. Exp Cell Res 364:175-183 (2018). PubMed: 29408206
- Zhu Y et al. Identification of biomarker microRNAs for predicting the response of colorectal cancer to neoadjuvant chemoradiotherapy based on microRNA regulatory network. Oncotarget 8:2233-2248 (2017). WB ; Human . PubMed: 27903980
- Wani N et al. C-X-C motif chemokine 12/C-X-C chemokine receptor type 7 signaling regulates breast cancer growth and metastasis by modulating the tumor microenvironment. Breast Cancer Res 16:R54 (2014). PubMed: 24886617