Overview

  • Product name

    Anti-GPCR RDC1/CXCR-7 antibody [EPR9321]
    See all GPCR RDC1/CXCR-7 primary antibodies
  • Description

    Rabbit monoclonal [EPR9321] to GPCR RDC1/CXCR-7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GPCR RDC1/CXCR-7 aa 300-400 (C terminal).

  • Positive control

    • THP-1, K562, Raji, C6, Raw 264.7, and NIH3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9321
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
    • Tissue specificity

      Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACKR3 antibody
      • atypical chemokine receptor 3 antibody
      • C X C chemokine receptor type 7 antibody
      • C-X-C chemokine receptor type 7 antibody
      • Chemokine (C-X-C motif) receptor 7 antibody
      • Chemokine C X C motif receptor 7 antibody
      • Chemokine orphan receptor 1 antibody
      • CMKOR1 antibody
      • CXC R7 antibody
      • CXC-R7 antibody
      • CXCR 7 antibody
      • CXCR-7 antibody
      • CXCR7 antibody
      • CXCR7_HUMAN antibody
      • G protein coupled receptor 159 antibody
      • G protein coupled receptor antibody
      • G protein coupled receptor RDC1 homolog antibody
      • G-protein coupled receptor 159 antibody
      • G-protein coupled receptor RDC1 homolog antibody
      • GPCR RDC1 antibody
      • GPR159 antibody
      • GPRN1 antibody
      • RDC 1 antibody
      • RDC-1 antibody
      • RDC1 antibody
      see all

    Images

    • All lanes : Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution (purified)

      Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 41 kDa



      Blocking and diluting buffer : 5% NFDM/TBST
    • Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/10000 dilution (purified) + THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 41 kDa



      Blocking and diluting buffer : 5% NFDM/TBST
    • Overlay histogram showing THP1 cells stained with unpurified ab138509 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab138509, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • All lanes : Anti-GPCR RDC1/CXCR-7 antibody [EPR9321] (ab138509) at 1/1000 dilution (unpurified)

      Lane 1 : THP-1 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw 264.7 cell lysate
      Lane 6 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    References

    This product has been referenced in:

    • Yu L  et al. Transcriptional and translational-uncoupling in regulation of the CXCL12 and its receptors CXCR4, 7 in THP-1 monocytes and macrophages. Immun Inflamm Dis 6:106-116 (2018). Read more (PubMed: 29105376) »
    • Zhu Y  et al. Identification of biomarker microRNAs for predicting the response of colorectal cancer to neoadjuvant chemoradiotherapy based on microRNA regulatory network. Oncotarget 8:2233-2248 (2017). WB ; Human . Read more (PubMed: 27903980) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Peritoneal Cells)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    20 µg
    Specification
    Peritoneal Cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 17 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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