Overview

  • Product name
    Anti-GPCR TAS1R1 antibody - N-terminal
    See all GPCR TAS1R1 primary antibodies
  • Description
    Rabbit polyclonal to GPCR TAS1R1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 129-223 of Human GPCR TAS1R1 (UniProt ID: Q7RTX1).

  • Positive control
    • A549, HeLa, Jurkat and NT2D1 cell lysates; HeLa cells; Human CL1-0 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • G protein coupled receptor 70 antibody
    • G protein-coupled receptor 70 antibody
    • G-protein coupled receptor 70 antibody
    • gm 148 antibody
    • gm148 antibody
    • GPR 70 antibody
    • GPR70 antibody
    • Krueppel related zinc finger protein 3 (HKR3 protein) antibody
    • seven transmembrane helix receptor antibody
    • Sweet taste receptor T1r antibody
    • T1R1 antibody
    • TAS1 R1 antibody
    • TAS1R1 antibody
    • Taste receptor type 1 member 1 antibody
    • taste receptor, type 1, member 1 antibody
    • TR 1 antibody
    • TR1 antibody
    • TS1R1_HUMAN antibody
    see all

Images

  • Anti-GPCR TAS1R1 antibody - N-terminal (ab155143) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 93 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human CL1-0 xenograft tissue, labeling GPCR TAS1R1 using ab155143 at a 1/100 dilution.
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling GPCR TAS1R1 using ab155143 at a 1/200 dilution. Lower image costained with Hoerchst 33342.

References

This product has been referenced in:
  • Zhou Y  et al. Methionine Regulates mTORC1 via the T1R1/T1R3-PLCß-Ca(2+)-ERK1/2 Signal Transduction Process in C2C12 Cells. Int J Mol Sci 17:N/A (2016). WB ; Mouse . Read more (PubMed: 27727170) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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