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Rabbit polyclonal to GPCR TAS1R1 - N-terminal
Synthetic peptide, corresponding to a region within N terminal amino acids 129-223 of Human GPCR TAS1R1 (UniProt ID: Q7RTX1).
A549, HeLa, Jurkat and NT2D1 cell lysates; HeLa cells; Human CL1-0 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000.
Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
Information by UniProt
G protein coupled receptor 70 antibody
G protein-coupled receptor 70 antibody
G-protein coupled receptor 70 antibody
Western blot - Anti-GPCR TAS1R1 antibody - N-terminal (ab155143)
Anti-GPCR TAS1R1 antibody - N-terminal (ab155143) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 93 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR TAS1R1 antibody - N-terminal (ab155143)
Immunohistochemical analysis of paraffin embedded Human CL1-0 xenograft tissue, labeling GPCR TAS1R1 using ab155143 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-GPCR TAS1R1 antibody - N-terminal (ab155143)
Immunofluorescence analysis of methanol fixed HeLa cells, labeling GPCR TAS1R1 using ab155143 at a 1/200 dilution. Lower image costained with Hoerchst 33342.
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