Overview

  • Product name

  • Description

    Goat polyclonal to GPCR TAS1R2
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Synthetic peptide:

    CSKRCQSGQKKKP

    , corresponding to internal sequence amino acids 495-507 of Human GPCR TAS1R2 (NP_689418.2)

  • Positive control

    • Human Pancreas lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Putative taste receptor. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • G protein coupled receptor 71 antibody
      • G-protein coupled receptor 71 antibody
      • GPR71 antibody
      • Sweet taste receptor T1R2 antibody
      • T1R2 antibody
      • TAS1R2 antibody
      • Taste receptor type 1 member 2 antibody
      • TR2 antibody
      • TS1R2_HUMAN antibody
      see all

    Images

    • Anti-GPCR TAS1R2 antibody (ab77346) at 0.3 µg/ml + Human pancreas lysate in RIPA buffer at 35 µg

      Predicted band size: 95 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    This product has been referenced in:

    • Lee RJ  et al. Bitter and sweet taste receptors regulate human upper respiratory innate immunity. J Clin Invest 124:1393-405 (2014). Read more (PubMed: 24531552) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Duodenum)
    Specification
    Duodenum
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C

    Ms. Bridgette Chia

    Verified customer

    Submitted Jul 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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