Key features and details
- Rabbit polyclonal to GPCR TGR5
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-GPCR TGR5 antibody
See all GPCR TGR5 primary antibodies
DescriptionRabbit polyclonal to GPCR TGR5
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region between residues 250 and 300 of human GPCR TGR5 (NP_733800.1).
General notesGPCR is multi pass membrane protein, customers may try heating the lysates for 15-20 minutes at 60-70C instead of boiling the samples. Non fat dry milk as blocking agent seems effective than BSA with current lot. Please contact technical support team for more information.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab72608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).|
FunctionReceptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
Tissue specificityUbiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon, cecum and liver, but not in esophagus and rectum.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- BG 37 antibody
- BG37 antibody
- G protein coupled bile acid receptor 1 antibody
Lane 2 : Anti-GPCR TGR5 antibody (ab72608) at 1/1000 dilution
Lanes 3-4 : Anti-GPCR TGR5 antibody (ab72608) at 1/5000 dilution
Lane 1 : Pre-immune serum
Lane 2 : Human spleen lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat spleen lysate
Predicted band size: 35 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.
Formalin-fixed, paraffin-embedded mouse colon tissue stained for GPCR TGR5 with ab72608 at 1/200 dilution in immunohistochemical analysis. DAB staining. Counter stained with Hematoxylin.
HEK-293 (human epithelial cell line from embryonic kidney) cells stained for GPCR TGR5 (green) using ab72608 at 1/50 dilution ICC/IF.
The nuclear counter stain is DAPI (blue). Alpha- tubulin is stained with Dylight 550 (red).
IHC image of ab72608 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72608, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab72608 has been referenced in 16 publications.
- Li CL et al. Smoking as an Independent Risk Factor for Hepatocellular Carcinoma Due to the a7-Nachr Modulating the JAK2/STAT3 Signaling Axis. J Clin Med 8:N/A (2019). PubMed: 31492006
- Li ZY et al. Activation of TGR5 alleviates inflammation in rheumatoid arthritis peripheral blood mononuclear cells and in mice with collagen II-induced arthritis. Mol Med Rep 20:4540-4550 (2019). PubMed: 31702035
- Dumas SN & Ntambi JM Increased hydrophilic plasma bile acids are correlated with protection from adiposity in skin-specific stearoyl-CoA desaturase-1 deficient mice. PLoS One 13:e0199682 (2018). PubMed: 29965978
- Carino A et al. The bile acid receptor GPBAR1 (TGR5) is expressed in human gastric cancers and promotes epithelial-mesenchymal transition in gastric cancer cell lines. Oncotarget 7:61021-61035 (2016). PubMed: 27409173
- Zhu J et al. Hydrophobic bile acids relax rat detrusor contraction via inhibiting the opening of the Na?/Ca²? exchanger. Sci Rep 6:21358 (2016). WB . PubMed: 26892434