Recombinant
Recombinant Anti-GPCR TGR7 antibody [EPR10597] (ab155099)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-GPCR TGR7 antibody [EPR10597]
See all GPCR TGR7 primary antibodies -
Description
Rabbit monoclonal [EPR10597] to GPCR TGR7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide, corresponding to residues in Human GPCR TGR7 (UniProt: Q8TDS7).
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Positive control
- Human testis, bladder, muscle and fetal heart lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10597 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.Can be blocked with GPCR TGR7 peptide (ab218717). |
Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
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Function
May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i). -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Mas subfamily. -
Cellular localization
Cell membrane. Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine. - Information by UniProt
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Database links
- Entrez Gene: 116512 Human
- Entrez Gene: 211578 Mouse
- Entrez Gene: 293648 Rat
- SwissProt: Q8TDS7 Human
- SwissProt: Q91ZB8 Mouse
- SwissProt: Q7TN41 Rat
- Unigene: 527802 Human
- Unigene: 370299 Mouse
see all -
Alternative names
- Beta alanine receptor antibody
- Beta-alanine receptor antibody
- G protein coupled receptor TGR7 antibody
see all
Images
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![Western blot - Anti-GPCR TGR7 antibody [EPR10597] (ab155099)](https://www.abcam.com/ps/products/155/ab155099/Images/ab155099-162016-anti-gpcr-tgr7-antibody-epr10597-western-blot.jpg)
Western blot - Anti-GPCR TGR7 antibody [EPR10597] (ab155099)All lanes : Anti-GPCR TGR7 antibody [EPR10597] (ab155099) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human bladder lysate
Lane 3 : Human muscle lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhou C et al. Expression and localization of MrgprD in mouse intestinal tract. Cell Tissue Res N/A:N/A (2019). Read more (PubMed: 30919047) »
