Anti-GPCR TGR7 antibody [EPR10597] (ab155099)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-GPCR TGR7 antibody [EPR10597]
See all GPCR TGR7 primary antibodies -
DescriptionRabbit monoclonal [EPR10597] to GPCR TGR7
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide, corresponding to residues in Human GPCR TGR7 (UniProt: Q8TDS7).
-
Positive control
- Human testis, bladder, muscle and fetal heart lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20ºC.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR10597
-
IsotypeIgG
-
Research areas
Associated products
-
Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab155099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.Can be blocked with GPCR TGR7 peptide (ab218717). |
Target
-
FunctionMay regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).
-
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Mas subfamily.
-
Cellular localizationCell membrane. Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine.
- Information by UniProt
-
Database links
- Entrez Gene: 116512 Human
- Entrez Gene: 211578 Mouse
- Entrez Gene: 293648 Rat
- SwissProt: Q8TDS7 Human
- SwissProt: Q91ZB8 Mouse
- SwissProt: Q7TN41 Rat
- Unigene: 527802 Human
- Unigene: 370299 Mouse
see all -
Alternative names
- Beta alanine receptor antibody
- Beta-alanine receptor antibody
- G protein coupled receptor TGR7 antibody
see all
Images
-
![Western blot - Anti-GPCR TGR7 antibody [EPR10597] (ab155099)](https://www.abcam.com/ps/datasheet/images/155/ab155099/GPCR-TGR7-Primary-antibodies-ab155099-1.jpg)
Western blot - Anti-GPCR TGR7 antibody [EPR10597] (ab155099)All lanes : Anti-GPCR TGR7 antibody [EPR10597] (ab155099) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human bladder lysate
Lane 3 : Human muscle lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDa
Protocols
References
ab155099 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"