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Rabbit polyclonal to GPLD1
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human GPLD1.
(Peptide available as
This antibody gave a positive signal in Human and Mouse Plasma Total Protein Preparations.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Belongs to the GPLD1 family.
Contains 7 FG-GAP repeats.
Information by UniProt
Glycoprotein phospholipase D antibody
Glycosyl-phosphatidylinositol-specific phospholipase D antibody
Western blot - Anti-GPLD1 antibody (ab82330)
All lanes :
Anti-GPLD1 antibody (ab82330) at 1 µg/ml
Lane 1 :
Human Plasma Total Protein Lysate
Lane 2 :
Mouse Plasma Total Protein Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
120 kDa (
why is the actual band size different from the predicted?
The GPLD1 protein has a predicted molecular weight of 92 kDa, however it has several potential glycosylation sites (SwissProt data) that might explain its migration at a higher MW.
Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"