Overview

  • Product name
  • Description
    Rabbit polyclonal to GPLD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human GPLD1.

    (Peptide available as ab92422.)

  • Positive control
    • This antibody gave a positive signal in Human and Mouse Plasma Total Protein Preparations.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
    • Sequence similarities
      Belongs to the GPLD1 family.
      Contains 7 FG-GAP repeats.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glycoprotein phospholipase D antibody
      • Glycosyl-phosphatidylinositol-specific phospholipase D antibody
      • GPI-PLD antibody
      • GPI-specific phospholipase D antibody
      • GPIPLD antibody
      • GPIPLDM antibody
      • GPLD1 antibody
      • MGC22590 antibody
      • PHLD_HUMAN antibody
      • Phosphatidylinositol glycan specific phospholipase D antibody
      • Phosphatidylinositol-glycan-specific phospholipase D antibody
      • PI-G PLD antibody
      • PIGPLD antibody
      • PIGPLD1 antibody
      see all

    Images

    • All lanes : Anti-GPLD1 antibody (ab82330) at 1 µg/ml

      Lane 1 : Human Plasma Total Protein Lysate
      Lane 2 : Mouse Plasma Total Protein Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)


      Exposure time: 5 minutes


      The GPLD1 protein has a predicted molecular weight of 92 kDa, however it has several potential glycosylation sites (SwissProt data) that might explain its migration at a higher MW. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    References

    ab82330 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82330.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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