Overview

  • Product name

    Anti-GPNMB antibody [EPR22011-11]
    See all GPNMB primary antibodies
  • Description

    Rabbit monoclonal [EPR22011-11] to GPNMB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GPNMB aa 1-300. The exact sequence is proprietary.
    Database link: Q14956

  • Positive control

    • WB: SK-MEL-28 whole cell lysate. IHC-P: Human melanoma and colonic carcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22011-11
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/1000.

Target

  • Function

    Could be a melanogenic enzyme.
  • Tissue specificity

    Not restricted to the melanocytic lineage.
  • Sequence similarities

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • Developmental stage

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycoprotein (transmembrane) nmb antibody
    • Glycoprotein nmb antibody
    • Glycoprotein nmb like protein antibody
    • Gpnmb antibody
    • GPNMB_HUMAN antibody
    • HGFIN antibody
    • NMB antibody
    • Osteoactivin antibody
    • Transmembrane glycoprotein antibody
    • Transmembrane glycoprotein HGFIN antibody
    • Transmembrane glycoprotein NMB antibody
    see all

Images

  • All lanes : Anti-GPNMB antibody [EPR22011-11] (ab222109) at 1/1000 dilution

    Lanes 1-2 : SK-MEL-28 (human malignant melanoma) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa
    Observed band size: 120,95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 81 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    Negative control: HeLa (PMID: 20056711)

  • Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling GPNMB with ab222109 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in tumor cells of human melanoma (PMID: 19320736). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling GPNMB with ab222109 at 1/1000 dilution, followed by aGoat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stroma cells of human colonic carcinoma (PMID: 20215530). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

References

ab222109 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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