Overview

  • Product name

    Anti-GPNMB antibody [EPR22011-47]
    See all GPNMB primary antibodies
  • Description

    Rabbit monoclonal [EPR22011-47] to GPNMB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GPNMB aa 1-300. The exact sequence is proprietary.
    Database link: Q14956

  • Positive control

    • WB: SK-MEL-28 whole cell lysate. ICC/IF: SK-MEL-28 cells. Flow Cyt: SK-MEL-28 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22011-47
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 64 kDa).
Flow Cyt 1/500.
ICC/IF 1/500.

Target

  • Function

    Could be a melanogenic enzyme.
  • Tissue specificity

    Not restricted to the melanocytic lineage.
  • Sequence similarities

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • Developmental stage

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycoprotein (transmembrane) nmb antibody
    • Glycoprotein nmb antibody
    • Glycoprotein nmb like protein antibody
    • Gpnmb antibody
    • GPNMB_HUMAN antibody
    • HGFIN antibody
    • NMB antibody
    • Osteoactivin antibody
    • Transmembrane glycoprotein antibody
    • Transmembrane glycoprotein HGFIN antibody
    • Transmembrane glycoprotein NMB antibody
    see all

Images

  • All lanes : Anti-GPNMB antibody [EPR22011-47] (ab235873) at 1/1000 dilution

    Lanes 1-2 : SK-MEL-28 (human malignant melanoma) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 64 kDa
    Observed band size: 120,95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).

    Negative control: HeLa (PMID: 20056711).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (human malignant melanoma) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GPNMB with ab235873 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining in SK-MEL-28 cell line.

    Negative control: HeLa (PMID: 20056711.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left panel)  and SK-MEL-28 (human malignant melanoma, Right panel) labeling GPNMB with ab235873 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 20056711).

References

ab235873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235873.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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