Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22011-47] to GPNMB
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-GPNMB antibody [EPR22011-47]
See all GPNMB primary antibodies
DescriptionRabbit monoclonal [EPR22011-47] to GPNMB
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human GPNMB aa 1-300. The exact sequence is proprietary.
Database link: Q14956
- WB: SK-MEL-28 whole cell lysate. ICC/IF: SK-MEL-28 cells. Flow Cyt: SK-MEL-28 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab235873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 64 kDa).|
FunctionCould be a melanogenic enzyme.
Tissue specificityNot restricted to the melanocytic lineage.
Sequence similaritiesBelongs to the PMEL/NMB family.
Contains 1 PKD domain.
Developmental stageExpression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Glycoprotein (transmembrane) nmb antibody
- Glycoprotein nmb antibody
- Glycoprotein nmb like protein antibody
All lanes : Anti-GPNMB antibody [EPR22011-47] (ab235873) at 1/1000 dilution
Lanes 1-2 : SK-MEL-28 (human malignant melanoma) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 64 kDa
Observed band size: 120,95 kDa why is the actual band size different from the predicted?
Exposure time: 26 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 19320736).
Negative control: HeLa (PMID: 20056711).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (human malignant melanoma) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GPNMB with ab235873 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in SK-MEL-28 cell line.
Negative control: HeLa （PMID: 20056711.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left panel) and SK-MEL-28 (human malignant melanoma, Right panel) labeling GPNMB with ab235873 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 20056711).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235873 has not yet been referenced specifically in any publications.