Recombinant
RabMAb

Recombinant Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

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Overview

  • Product name

    Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free
    See all GPNMB primary antibodies

  • Description

    Rabbit monoclonal [EPR22011-47] to GPNMB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GPNMB aa 1-300. The exact sequence is proprietary.
    Database link: Q14956

  • Positive control

    • ICC/IF: SK-MEL-28 cells.

  • General notes

    Ab236211 is the carrier-free version of ab235873. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 64 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Could be a melanogenic enzyme.

  • Tissue specificity

    Not restricted to the melanocytic lineage.

  • Sequence similarities

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.

  • Developmental stage

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.

  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glycoprotein (transmembrane) nmb antibody
      • Glycoprotein nmb antibody
      • Glycoprotein nmb like protein antibody
      • Gpnmb antibody
      • GPNMB_HUMAN antibody
      • HGFIN antibody
      • NMB antibody
      • Osteoactivin antibody
      • Transmembrane glycoprotein antibody
      • Transmembrane glycoprotein HGFIN antibody
      • Transmembrane glycoprotein NMB antibody
      see all

    Images

    • Flow Cytometry - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)
      Flow Cytometry - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left panel)  and SK-MEL-28 (human malignant melanoma, Right panel) labeling GPNMB with ab235873 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: HeLa (PMID: 20056711).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235873).

    • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)
      Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (human malignant melanoma) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GPNMB with ab235873 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining in SK-MEL-28 cell line.

      Negative control: HeLa （PMID: 20056711.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235873).

    References

    ab236211 has not yet been referenced specifically in any publications.

    Publishing research using ab236211? Please let us know so that we can cite the reference in this datasheet.

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