RecombinantRabMAb

Recombinant Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22011-47] to GPNMB - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free
    See all GPNMB primary antibodies

  • Description

    Rabbit monoclonal [EPR22011-47] to GPNMB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GPNMB aa 1-300. The exact sequence is proprietary.
    Database link: Q14956

  • Positive control

    • ICC/IF: SK-MEL-28 cells.

  • General notes

    Ab236211 is the carrier-free version of ab235873. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab236211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 95, 120 kDa (predicted molecular weight: 64 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Could be a melanogenic enzyme.

  • Tissue specificity

    Not restricted to the melanocytic lineage.

  • Sequence similarities

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.

  • Developmental stage

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.

  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glycoprotein (transmembrane) nmb antibody
      • Glycoprotein nmb antibody
      • Glycoprotein nmb like protein antibody
      • Gpnmb antibody
      • GPNMB_HUMAN antibody
      • HGFIN antibody
      • NMB antibody
      • Osteoactivin antibody
      • Transmembrane glycoprotein antibody
      • Transmembrane glycoprotein HGFIN antibody
      • Transmembrane glycoprotein NMB antibody
      see all

    Images

    • Flow Cytometry - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)
      Flow Cytometry - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left panel)  and SK-MEL-28 (human malignant melanoma, Right panel) labeling GPNMB with ab235873 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: HeLa (PMID: 20056711).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235873).

    • Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)
      Immunocytochemistry/ Immunofluorescence - Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (human malignant melanoma) and HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GPNMB with ab235873 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing cytoplasmic staining in SK-MEL-28 cell line.

      Negative control: HeLa （PMID: 20056711.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235873).

    • Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)
      Anti-GPNMB antibody [EPR22011-47] - BSA and Azide free (ab236211)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab236211? Please let us know so that we can cite the reference in this datasheet.

    ab236211 has not yet been referenced specifically in any publications.

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