Overview

  • Product name

    Anti-GPNMB antibody [SP299]
    See all GPNMB primary antibodies
  • Description

    Rabbit monoclonal [SP299] to GPNMB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GPNMB aa 1-500. The exact sequence is proprietary.
    Database link: Q14956

  • Positive control

    • WB: A-375 cell lysate. Flow Cyt: A-375 cells. IHC-P: Human tonsil, reactive lymph node, lung, colon, lung squamous cell carcinoma and cervical squamous cell carcinoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP299
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Primary incubation for 30 minutes at 4°C.

IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

WB 1/100. Predicted molecular weight: 64 kDa.

Primary incubation for 1 hour at room temperature.

Target

  • Function

    Could be a melanogenic enzyme.
  • Tissue specificity

    Not restricted to the melanocytic lineage.
  • Sequence similarities

    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • Developmental stage

    Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glycoprotein (transmembrane) nmb antibody
    • Glycoprotein nmb antibody
    • Glycoprotein nmb like protein antibody
    • Gpnmb antibody
    • GPNMB_HUMAN antibody
    • HGFIN antibody
    • NMB antibody
    • Osteoactivin antibody
    • Transmembrane glycoprotein antibody
    • Transmembrane glycoprotein HGFIN antibody
    • Transmembrane glycoprotein NMB antibody
    see all

Images

  • Anti-GPNMB antibody [SP299] (ab227695) at 1/100 dilution + A-375 (human malignant melanoma cell line) whole cell lysate

    Predicted band size: 64 kDa

  • Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells, labeling GPNMB with ab227695 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for GPNMB using ab227695 at 1/100 dilution in immunohistochemical analysis.

References

ab227695 has not yet been referenced specifically in any publications.

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