GppNHp, Non-hydrolyzable GTP analog (ab146659)

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Overview

  • Product name

    GppNHp, Non-hydrolyzable GTP analog

  • Description

    Non-hydrolyzable GTP analog

  • Biological description

    Non-hydrolyzable GTP analog. Has an amine group in place of an oxygen atom. Potent adenylate cyclase stimulator. Binds tightly to G-protein in the presence of Mg2+. Forms stable Ras complexes.

  • Purity

    > 95%

  • CAS Number

    64564-03-0

  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    [[(2R,3S,4R,5R)-5-(2-Amino-6-oxo-1H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-(phosphonatoamino)phosphinate tetralithium

  • Molecular weight

    545.93

  • Molecular formula

    C10H13Li4N6O13P3

  • PubChem identifier

    136494784

  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.

  • Solubility overview

    Soluble in water to 75 mM

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    [Li+].[Li+].[Li+].[Li+].C1=NC2=C(N1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)([O-])OP(=O)(NP(=O)([O-])[O-])[O-])O)O)N=C(NC2=O)N

  • Source

    Synthetic

References

This product has been referenced in:

  • Fetics SK  et al. Allosteric effects of the oncogenic RasQ61L mutant on Raf-RBD. Structure 23:505-16 (2015). Read more (PubMed: 25684575) »
  • Holzapfel G  et al. Shift in the equilibrium between on and off states of the allosteric switch in Ras-GppNHp affected by small molecules and bulk solvent composition. Biochemistry 51:6114-26 (2012). Read more (PubMed: 22845804) »
  • Spoerner M  et al. Conformational states of Ras complexed with the GTP analogue GppNHp or GppCH2p: implications for the interaction with effector proteins. Biochemistry 44:2225-36 (2005). Read more (PubMed: 15697248) »
See 1 Publication for this product

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