GppNHp, Non-hydrolyzable GTP analog (ab146659)
Key features and details
- Non-hydrolyzable GTP analog
- CAS Number: 64564-03-0
- Purity: > 95%
Soluble in water to 75 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
GppNHp, Non-hydrolyzable GTP analog -
Description
Non-hydrolyzable GTP analog -
Biological description
Non-hydrolyzable GTP analog. Has an amine group in place of an oxygen atom. Potent adenylate cyclase stimulator. Binds tightly to G-protein in the presence of Mg2+. Forms stable Ras complexes. -
Purity
> 95% -
CAS Number
64564-03-0 -
Chemical structure
Properties
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Chemical name
[[(2R,3S,4R,5R)-5-(2-Amino-6-oxo-1H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-(phosphonatoamino)phosphinate tetralithium
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Molecular weight
545.93 -
Molecular formula
C10H13Li4N6O13P3 -
PubChem identifier
136494784 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 75 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Li+].[Li+].[Li+].[Li+].C1=NC2=C(N1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)([O-])OP(=O)(NP(=O)([O-])[O-])[O-])O)O)N=C(NC2=O)N -
Source
Synthetic
Associated products
Images
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Chemical Structure - GppNHp, Non-hydrolyzable GTP analog (ab146659)2D chemical structure image of ab146659, GppNHp, Non-hydrolyzable GTP analog
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab146659 has been referenced in 6 publications.
- Lattao R et al. Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins. Dev Cell N/A:N/A (2021). PubMed: 33725482
- Kutsch M et al. Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions. EMBO J 39:e104926 (2020). PubMed: 32510692
- English JG et al. VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells. Cell 178:748-761.e17 (2019). PubMed: 31280962
- Kanie T & Jackson PK Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP. Bio Protoc 8:N/A (2018). PubMed: 29951569
- Ivanova AA et al. Biochemical characterization of purified mammalian ARL13B protein indicates that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF). J Biol Chem 292:11091-11108 (2017). PubMed: 28487361
- Kanie T et al. The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Dev Cell 42:22-36.e12 (2017). PubMed: 28625565