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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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RecombinantRabMAb

Recombinant Anti-GPR 139 antibody [EPR10766] (ab155983)

  • Datasheet
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Western blot - Anti-GPR 139 antibody [EPR10766] (ab155983)
  • Anti-GPR 139 antibody [EPR10766] (ab155983)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10766] to GPR 139
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-GPR 139 antibody [EPR10766]
    See all GPR 139 primary antibodies
  • Description

    Rabbit monoclonal [EPR10766] to GPR 139
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human GPR 139 (UniProt ID: Q6DWJ6).

  • Positive control

    • U-87 MG, SH SY5Y and HeLa cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10766
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Alternative Versions

    • Anti-GPR 139 antibody [EPR10766] - BSA and Azide free (ab249228)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab155983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Orphan receptor. Seems to act through a G(q/11)-mediated pathway.
    • Tissue specificity

      Expressed almost exclusively in the brain. Detected at very low levels in the peripheral tissues.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q6DWJ6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 124274 Human
      • Entrez Gene: 209776 Mouse
      • Entrez Gene: 293545 Rat
      • SwissProt: Q6DWJ6 Human
      • SwissProt: Q80UC8 Mouse
      • SwissProt: P0C0W8 Rat
      • Unigene: 446875 Human
      • Unigene: 300632 Mouse
      • Unigene: 215835 Rat
      see all
    • Alternative names

      • G coupled orphan receptor GPRg1 antibody
      • G protein coupled receptor PGR3 antibody
      • G protein-coupled receptor 139 antibody
      • G(q)-coupled orphan receptor GPRg1 antibody
      • G-protein-coupled receptor PGR3 antibody
      • GP139_HUMAN antibody
      • GPCR 139 antibody
      • GPCR139 antibody
      • Gpr139 antibody
      • GPRG1 antibody
      • PGR3 antibody
      • Probable G-protein coupled receptor 139 antibody
      see all

    Images

    • Western blot - Anti-GPR 139 antibody [EPR10766] (ab155983)
      Western blot - Anti-GPR 139 antibody [EPR10766] (ab155983)
      All lanes : Anti-GPR 139 antibody [EPR10766] (ab155983) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 41 kDa

    • Anti-GPR 139 antibody [EPR10766] (ab155983)
      Anti-GPR 139 antibody [EPR10766] (ab155983)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab155983? Please let us know so that we can cite the reference in this datasheet.

    ab155983 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question


    I would like to know if the epitope that this antibody is targeting is extracellular?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 02 2014

    Answer



    I have compared the immunogen sequence to the primary structure of GPR139 and find that ab155983 will target an extracellular epitope.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Jun 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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