Recombinant Anti-GPR 139 antibody [EPR10766] - BSA and Azide free (ab249228)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10766] to GPR 139 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GPR 139 antibody [EPR10766] - BSA and Azide free
See all GPR 139 primary antibodies -
Description
Rabbit monoclonal [EPR10766] to GPR 139 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249228 is the carrier-free version of ab155983.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10766 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
Target
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Function
Orphan receptor. Seems to act through a G(q/11)-mediated pathway. -
Tissue specificity
Expressed almost exclusively in the brain. Detected at very low levels in the peripheral tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 124274 Human
- Entrez Gene: 209776 Mouse
- Entrez Gene: 293545 Rat
- SwissProt: Q6DWJ6 Human
- SwissProt: Q80UC8 Mouse
- SwissProt: P0C0W8 Rat
- Unigene: 446875 Human
- Unigene: 300632 Mouse
see all -
Alternative names
- G coupled orphan receptor GPRg1 antibody
- G protein coupled receptor PGR3 antibody
- G protein-coupled receptor 139 antibody
see all
Images
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All lanes : Anti-GPR 139 antibody [EPR10766] (ab155983) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDaThis data was developed using ab155983, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249228 has not yet been referenced specifically in any publications.