Anti-GPR 174 antibody (ab92796)
Key features and details
- Rabbit polyclonal to GPR 174
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPR 174 antibody
See all GPR 174 primary antibodies -
Description
Rabbit polyclonal to GPR 174 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the internal region of Human GPR 174.
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Positive control
- Extract from K562 cells. LOVO cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using an epitope specific immunogen -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Putative receptor for purines coupled to G-proteins. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 84636 Human
- SwissProt: Q9BXC1 Human
- Unigene: 326713 Human
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Alternative names
- FKSG79 antibody
- G protein-coupled receptor 174 antibody
- GP174_HUMAN antibody
see all
Images
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All lanes : Anti-GPR 174 antibody (ab92796) at 1/500 dilution
Lane 1 : Extract from K562 cells with no blocking peptide
Lane 2 : Extract from K562 cells with blocking peptide
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa -
ab92796 at 1/500 dilution staining GPR 174 in LOVO cells (1) without blocking peptide and (2) with blocking peptide, by immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92796 has been referenced in 1 publication.
- Feng B et al. Upregulation of Extracellular Vesicles-Encapsulated miR-132 Released From Mesenchymal Stem Cells Attenuates Ischemic Neuronal Injury by Inhibiting Smad2/c-jun Pathway via Acvr2b Suppression. Front Cell Dev Biol 8:568304 (2020). PubMed: 33763412