Overview

  • Product name

    Anti-GPR158 antibody
  • Description

    Rabbit polyclonal to GPR158
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 914-1052 of Human GPR158.

  • Positive control

    • RT4 and U 251 MG cell lysates; Human gall bladder tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • All lanes : Anti-GPR158 antibody (ab121388) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique.


    10-20% SDS PAGE
  • ab121388 at 1/100 dilution staining GPR158 in Paraffin-emebedded Human gall bladder tissue by Immunohistochemistry showing weak nuclear positivity in glandular cells.

References

This product has been referenced in:

  • Li N  et al. Inhibition of GPR158 by microRNA-449a suppresses neural lineage of glioma stem/progenitor cells and correlates with higher glioma grades. Oncogene 37:4313-4333 (2018). Read more (PubMed: 29720725) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (mouse neural stem cells)
Specification
mouse neural stem cells
Permeabilization
Yes - tritonX
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 13 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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