Overview

  • Product name
  • Description
    Rabbit polyclonal to GPR161
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MSLNSSLSCR KELSNLTEEE GGEGGVIITQ FIAIIVITIF VCLGNLVIVV, corresponding to N terminal amino acids 1-50 of Human GPR161

  • Positive control
    • HepG2 cell lysate or human kidney tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • ICC/IF image of ab58679 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58679 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GPR161 antibody (ab58679) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 59 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPR161 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

This product has been referenced in:
  • Feigin ME  et al. G-protein-coupled receptor GPR161 is overexpressed in breast cancer and is a promoter of cell proliferation and invasion. Proc Natl Acad Sci U S A 111:4191-6 (2014). Human . Read more (PubMed: 24599592) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Mouse Cell (NIH3T3 cells)
Specification
NIH3T3 cells
Permeabilization
Yes - 0.2% Tween 20
Fixative
Paraformaldehyde

Dr. Xuecai Ge

Verified customer

Submitted Aug 09 2013

Answer

Thank you very much for your interest in ab58679.

To our knowledge, ab58679 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab58679 now, test it in Flow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab58679 in Flow Cytometry. I will then send a discount code. This code must be issued before purchasing ab58679 so please wait for my reply before ordering.

2. Purchase ab58679 either by phone, fax, or online (www.abcam.com).

3. Test it in Flow Cytometry.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN /PRODUCT] ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab58679 turns out to be unsuitable for Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up