Key features and details
- Rabbit polyclonal to GPR171
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GPR171 antibody
See all GPR171 primary antibodies
DescriptionRabbit polyclonal to GPR171
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- IHC-P: Human prostate tissue. ICC/IF: MCF7 cells. WB: K562 cell extract.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol
PBS is without Mg2+, Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 37 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- F730001G15Rik antibody
- G protein coupled receptor 171 antibody
- G protein coupled receptor H963 antibody
All lanes : Anti-GPR171 antibody (ab189282) at 1/500 dilution
Lane 1 : K562 cell extract with Without synthesized peptide.
Lane 2 : K562 cell extract with With synthesized peptide.
Predicted band size: 37 kDa
Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate tissue labeling GPR171 with ab189282 at 5 µg/ml.
Immunofluorescent analysis of MCF7 cells labeling GPR171 with ab189282 at 1/100 dilution (left). Right panel is treated with the immunizing peptide.
ab189282 has not yet been referenced specifically in any publications.