The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000.
Requires antigen retrieval - 100mM boiling Tris pH9.
Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
Immunohistochemical analysis of frozen section of E10.5 mouse telencephalic vessicle tissue, labeling GPR177 with ab176376 at 1/1000 dilution, followed by Alexafluor goat anti-rabbit secondary. Sections are co-stained with DAPI nuclear stain.
Tan SH et al. Wnts produced by Osterix-expressing osteolineage cells regulate their proliferation and differentiation. Proc Natl Acad Sci U S A111:E5262-71 (2014).
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