• Product name

  • Description

    Chicken polyclonal to GPR177/WLS
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GPR177/WLS aa 524-541 (C terminal).


  • Positive control

    • HEK293 cell lysate transfected with GPR177/WLS-GFP. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.
  • General notes

     This product was previously labelled as GPR177




Our Abpromise guarantee covers the use of ab72385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).


  • Function

    Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
  • Sequence similarities

    Belongs to the wntless family.
  • Cellular localization

    Golgi apparatus membrane. Cytoplasmic vesicle membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf139 antibody
    • DKFZp686I0788 antibody
    • Evenness interrupted, Drosophila, homolg of antibody
    • EVI antibody
    • FLJ23091 antibody
    • G protein coupled receptor 177 antibody
    • GPR 177 antibody
    • Integral membrane protein GPR177 antibody
    • MGC131760 antibody
    • MGC14878 antibody
    • mig-14 antibody
    • MRP antibody
    • Protein evenness interrupted homolog antibody
    • Protein wntless homolog antibody
    • Putative NF-kappa-B-activating protein 373 antibody
    • Putative NFkB activating protein 373 antibody
    • WLS antibody
    • WLS_HUMAN antibody
    • wntless homolog (Drosophila) antibody
    • Wntless homolog antibody
    • wntless Wnt ligand secretion mediator antibody
    • Wntless, Drosophila, homolog of antibody
    see all


  • ICC/IF image of ab72385 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72385 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GPR177/WLS antibody (ab72385) at 1/2000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : HEK293 cell lysate transfected with GPR177/WLS-GFP

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    The HEK293 cell protein lysate contains both transfected GPR177/WLS-GFP fusion protein (75 kDa) and endogenous GPR177/WLS protein (~62 kDa).


This product has been referenced in:

  • de Groot RE  et al. Retromer dependent recycling of the wnt secretion factor wls is dispensable for stem cell maintenance in the Mammalian intestinal epithelium. PLoS One 8:e76971 (2013). WB ; Mouse . Read more (PubMed: 24130821) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72385.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up