Overview

  • Product name

  • Description

    Chicken polyclonal to GPR177/WLS
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GPR177/WLS aa 524-541 (C terminal).
    Sequence:

    HVDGPTEIYKLTRKEAGE

  • Positive control

    • HEK293 cell lysate transfected with GPR177/WLS-GFP. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.
  • General notes

     This product was previously labelled as GPR177

     

Properties

Applications

Our Abpromise guarantee covers the use of ab72385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2000 - 1/5000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • Function

    Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.
  • Sequence similarities

    Belongs to the wntless family.
  • Cellular localization

    Golgi apparatus membrane. Cytoplasmic vesicle membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf139 antibody
    • DKFZp686I0788 antibody
    • Evenness interrupted, Drosophila, homolg of antibody
    • EVI antibody
    • FLJ23091 antibody
    • G protein coupled receptor 177 antibody
    • GPR 177 antibody
    • Integral membrane protein GPR177 antibody
    • MGC131760 antibody
    • MGC14878 antibody
    • mig-14 antibody
    • MRP antibody
    • Protein evenness interrupted homolog antibody
    • Protein wntless homolog antibody
    • Putative NF-kappa-B-activating protein 373 antibody
    • Putative NFkB activating protein 373 antibody
    • WLS antibody
    • WLS_HUMAN antibody
    • wntless homolog (Drosophila) antibody
    • Wntless homolog antibody
    • wntless Wnt ligand secretion mediator antibody
    • Wntless, Drosophila, homolog of antibody
    see all

Images

  • ICC/IF image of ab72385 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72385 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GPR177/WLS antibody (ab72385) at 1/2000 dilution

    Lane 1 : Molecular weight markers
    Lane 2 : HEK293 cell lysate transfected with GPR177/WLS-GFP

    Predicted band size: 62 kDa
    Observed band size: 62 kDa



    The HEK293 cell protein lysate contains both transfected GPR177/WLS-GFP fusion protein (75 kDa) and endogenous GPR177/WLS protein (~62 kDa).

References

This product has been referenced in:

  • de Groot RE  et al. Retromer dependent recycling of the wnt secretion factor wls is dispensable for stem cell maintenance in the Mammalian intestinal epithelium. PLoS One 8:e76971 (2013). WB ; Mouse . Read more (PubMed: 24130821) »
See 1 Publication for this product

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