Recombinant Anti-GPR182 antibody [EPR14327] (ab182555)
![Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)](https://www.abcam.com/ps/products/182/ab182555/Images/ab182555-213945-ab182555WB1.jpg "Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14327] to GPR182
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GPR182 antibody [EPR14327]
See all GPR182 primary antibodies -
Description
Rabbit monoclonal [EPR14327] to GPR182 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle and Human fetal heart lysates; HT-1376 and U-87 MG cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14327 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Orphan receptor. -
Tissue specificity
Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 11318 Human
- Entrez Gene: 11536 Mouse
- Omim: 605307 Human
- SwissProt: O15218 Human
- SwissProt: P43142 Mouse
- Unigene: 483909 Human
- Unigene: 2857 Mouse
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Alternative names
- 7TMR antibody
- ADMR antibody
- Adrenomedullin L1 receptor antibody
see all
Images
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Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
![Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)](https://www.abcam.com/ps/products/182/ab182555/Images/ab182555-213943-ab182555WB2.jpg)
Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
![Anti-GPR182 antibody [EPR14327] (ab182555)](https://www.abcam.com/ps/products/182/ab182555/Images/ab182555-3-benefits-of-recombinant-antibodies.png)
Anti-GPR182 antibody [EPR14327] (ab182555)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab182555 has been referenced in 1 publication.
- Xia J et al. A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33785593