RecombinantRabMAb

Recombinant Anti-GPR182 antibody [EPR14327] (ab182555)

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Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
  • Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
  • Anti-GPR182 antibody [EPR14327] (ab182555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14327] to GPR182
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-GPR182 antibody [EPR14327]
    See all GPR182 primary antibodies

  • Description

    Rabbit monoclonal [EPR14327] to GPR182

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle and Human fetal heart lysates; HT-1376 and U-87 MG cell lysates.

  • General notes

     

     

     This product was previously labelled as Adrenomedullin Receptor L1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    Orphan receptor.

  • Tissue specificity

    Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 7TMR antibody
      • ADMR antibody
      • Adrenomedullin L1 receptor antibody
      • Adrenomedullin receptor antibody
      • AM R antibody
      • AM-R antibody
      • AMR antibody
      • G protein coupled receptor 182 antibody
      • g protein-coupled receptor g10d antibody
      • G-protein coupled receptor 182 antibody
      • G10D antibody
      • Gamrh antibody
      • GP182_HUMAN antibody
      • GPR 182 antibody
      • GPR182 antibody
      • hAMR antibody
      • hrhAMR antibody
      • MGC34399 antibody
      • rAMR antibody
      see all

    Images

    • Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution

      Lane 1 : HT-1376 cell lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Anti-GPR182 antibody [EPR14327] (ab182555)
      Anti-GPR182 antibody [EPR14327] (ab182555)

    References (0)

    Publishing research using ab182555? Please let us know so that we can cite the reference in this datasheet.

    ab182555 has not yet been referenced specifically in any publications.

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