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    gpr182-antibody-epr14327-ab182555.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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RecombinantRabMAb

Recombinant Anti-GPR182 antibody [EPR14327] (ab182555)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
  • Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
  • Anti-GPR182 antibody [EPR14327] (ab182555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14327] to GPR182
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-GPR182 antibody [EPR14327] - BSA and Azide free (ab250614)

View more associated products

Overview

  • Product name

    Anti-GPR182 antibody [EPR14327]
    See all GPR182 primary antibodies
  • Description

    Rabbit monoclonal [EPR14327] to GPR182
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human skeletal muscle and Human fetal heart lysates; HT-1376 and U-87 MG cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14327
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Associated products

  • Alternative Versions

    • Anti-GPR182 antibody [EPR14327] - BSA and Azide free (ab250614)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    Orphan receptor.
  • Tissue specificity

    Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession O15218 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11318 Human
    • Entrez Gene: 11536 Mouse
    • Omim: 605307 Human
    • SwissProt: O15218 Human
    • SwissProt: P43142 Mouse
    • Unigene: 483909 Human
    • Unigene: 2857 Mouse
    • Alternative names

      • 7TMR antibody
      • ADMR antibody
      • Adrenomedullin L1 receptor antibody
      • Adrenomedullin receptor antibody
      • AM R antibody
      • AM-R antibody
      • AMR antibody
      • G protein coupled receptor 182 antibody
      • g protein-coupled receptor g10d antibody
      • G-protein coupled receptor 182 antibody
      • G10D antibody
      • Gamrh antibody
      • GP182_HUMAN antibody
      • GPR 182 antibody
      • GPR182 antibody
      • hAMR antibody
      • hrhAMR antibody
      • MGC34399 antibody
      • rAMR antibody
      see all

    Images

    • Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      Western blot - Anti-GPR182 antibody [EPR14327] (ab182555)
      All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution

      Lane 1 : HT-1376 cell lysate
      Lane 2 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    • Anti-GPR182 antibody [EPR14327] (ab182555)
      Anti-GPR182 antibody [EPR14327] (ab182555)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab182555? Please let us know so that we can cite the reference in this datasheet.

    ab182555 has been referenced in 1 publication.

    • Xia J  et al. A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33785593

    Customer reviews and Q&As

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