- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-GPR2/CCR10 antibody
See all GPR2/CCR10 primary antibodies -
DescriptionGoat polyclonal to GPR2/CCR10
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Host speciesGoat
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SpecificityPeptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
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Tested applicationsSuitable for: Flow Cyt, ELISA, ICC, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse
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Immunogen
Synthetic peptide:
KPTEQVSWGLYSGYDEEAYSVGPLPELC
, corresponding to amino acids 4-31 of Mouse CCR10. -
Positive control
- Mouse lymph nodes or small intestine.
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General notes
Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
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Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesReceptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. CCR10 in particular is the receptor for the skin-associated CC chemokine CCL27 (CTACK) as well as the novel chemokine CCL28 which displays chemotactic activity for resting CD4+ and CD8+ T cells.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5µg for 106 cells. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ELISA | 1/100000. | |
| ICC | 1/500. | |
| WB | 1/1000. | |
| IHC-P | 1/250. |
Target
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FunctionReceptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line.
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Tissue specificityExpressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 12777 Mouse
- SwissProt: Q9JL21 Mouse
- Unigene: 8021 Mouse
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Alternative names
- C C chemokine receptor type 10 antibody
- C-C chemokine receptor type 10 antibody
- C-C CKR-10 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPR2/CCR10 antibody (ab1661)Immunohistochemistry using ab1661 on a section of mouse small intestine
Protocols
References
This product has been referenced in:
- Hu K et al. CCL19 and CCL28 augment mucosal and systemic immune responses to HIV-1 gp140 by mobilizing responsive immunocytes into secondary lymph nodes and mucosal tissue. J Immunol 191:1935-47 (2013). Read more (PubMed: 23858028) »
- Shankar SP et al. RGS16 attenuates pulmonary Th2/Th17 inflammatory responses. J Immunol 188:6347-56 (2012). ICC ; Mouse . Read more (PubMed: 22593615) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"