Anti-GPR2/CCR10 antibody (ab1661)

Thank you for your enquiry. The application of flow cytometry/FACS was not originally tested for this antibody. A customer purchased this antibody and wrote a review on the website stating that the antibody was used successfully for flow cytometry at a concentration of 0.5 ug/mL for 1 million cells. We have added this as an application because this customer used it successfully. I contacted the customer to try to find out what secondary antibody was used, and I was told he used an anti-goat PE conjugate of concentration: 0.5 ug/ml. We sell such an antibody, ab7004 a Donkey polyclonal to Goat IgG H&L (PE). I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Thank you for your e-mail. I would recommend the same steps as for frozen sections (long incubations, presence of triton in your buffers, checking the amplification kits work for other antibodies as these may go off). Finally you may wish to try a different method of antigen retrieval: try an enzymatic antigen retrieval method (you can find a protocol for that on our website under the resources tab). Please let me know if this helps and do not hesitate to contact me if you need further advice,

I'm sorry to hear you are having problems with ab1661. I would like to suggest some modifications to your protocol to help you detect CCR10 in your samples. -When fixing frozen sections, try ice-cold methanol/acetone for 10min. -check you permeabilise the cells by the presence of triton in your buffers (serum buffer and antibody buffer) (0.3% v/v) -incubate the sections in primary antibody at 4C overnight in a humidity chamber -incubate sections in secondary antibody for longer. Try 1-2 hours at 4C -check your secondary antibodies and other amplification kits work for other antibodies as these may go off. Finally, it is possible that during the dilution of your antibody to 1:10 and storage of this solution, the antibody has gone off. What buffer did you use? Did you add azide to prevent contaminations? I hope this information helps, please let me know if you still have problems, good luck with your immunohistochemistry.

Yes, ab7004 is an appropriate secondary antibody to use with ab1661. When you place your order, you can make your payment with a Mastercard or Visa credit card or you can use a Purchase Order number. An invoice will be mailed to you either the day of or day after shipment. If you need a proforma invoice we can send that to you as well. To place your order, please contact us either through phone, email, or fax: Abcam Ltd 21 Cambridge Science Park Milton Road Cambridge CB4 0TP UK Email: orders@abcam.com Phone:+44 1223 472030 Fax:+44 1223 472038 Office hours: 08.30 - 17.00 hrs (UK) Monday to Friday

To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Ab1661 is 383.00 Euro. The antibody is currently in stock and would be shipped to you by FedEx taking just a couple of days to reach you.